Primer Details for TRINITY_DN12372_c1_g8_i1 | |||
|---|---|---|---|
| (A)10gtggaaagacatgtataaaaacacctttctctcttttcaacatagccaaaagtaatat(A)10gaaaaagaaaaaaaggaaatcaacactccctcactttgttaaagaatcaaaaaaatg(A)23: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACAGTTTCTGTCATAAACCAAA | AGTGAGGGAGTGTTGATTTCCT | Start: 8 |
| Tm | 57.232 | 59.018 | End: 211 |
| Size | 24 | 22 | 204 |
| Primer 2 details | |||
| Sequence | AACACAGTTTCTGTCATAAACCAA | AGTGAGGGAGTGTTGATTTCCT | Start: 7 |
| Tm | 57.232 | 59.018 | End: 211 |
| Size | 24 | 22 | 205 |
| Primer 3 details | |||
| Sequence | ACACAGTTTCTGTCATAAACCAAAA | AGTGAGGGAGTGTTGATTTCCT | Start: 8 |
| Tm | 57.836 | 59.018 | End: 211 |
| Size | 25 | 22 | 204 |
| Primer 4 details | |||
| Sequence | AACACAGTTTCTGTCATAAACCAAA | AGTGAGGGAGTGTTGATTTCCT | Start: 7 |
| Tm | 57.836 | 59.018 | End: 211 |
| Size | 25 | 22 | 205 |
| Primer 5 details | |||
| Sequence | ACACAGTTTCTGTCATAAACCAAA | AGTGAGGGAGTGTTGATTTCCTT | Start: 8 |
| Tm | 57.232 | 59.604 | End: 211 |
| Size | 24 | 23 | 204 |
