Primer Details for TRINITY_DN12367_c1_g12_i1 |
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| (A)12g(A)15ccagaaaaaaaatatagaaaaaaacaaaaaaaaatccgattccccttaaagacaaggaaaacc(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGATTCCCCTTAAAGACAAGGA | TCCAACTTTTAAGGAAATCGGATCT | Start: 65 |
| Tm | 60.055 | 58.518 | End: 197 |
| Size | 23 | 25 | 133 |
| Primer 2 details |
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| Sequence | TCCGATTCCCCTTAAAGACAAGG | TCCAACTTTTAAGGAAATCGGATCT | Start: 64 |
| Tm | 60.055 | 58.518 | End: 197 |
| Size | 23 | 25 | 134 |
| Primer 3 details |
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| Sequence | CCGATTCCCCTTAAAGACAAGG | TCCAACTTTTAAGGAAATCGGATCT | Start: 65 |
| Tm | 58.721 | 58.518 | End: 197 |
| Size | 22 | 25 | 133 |
| Primer 4 details |
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| Sequence | CCGATTCCCCTTAAAGACAAGGA | TCCAACTTTTAAGGAAATCGGATC | Start: 65 |
| Tm | 60.055 | 57.247 | End: 197 |
| Size | 23 | 24 | 133 |
| Primer 5 details |
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| Sequence | TCCGATTCCCCTTAAAGACAAGG | TCCAACTTTTAAGGAAATCGGATC | Start: 64 |
| Tm | 60.055 | 57.247 | End: 197 |
| Size | 23 | 24 | 134 |
