ssr_primer
Primer Details for TRINITY_DN12363_c2_g2_i3 |
|---|
| (A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCACCCCATCTGAGAGGTA | GCAACTGGCGTTACTTGCAA | Start: 515 |
| Tm | 60.031 | 59.97 | End: 712 |
| Size | 20 | 20 | 198 |
| Primer 2 details |
|---|
| Sequence | CCCACCCCATCTGAGAGGTA | GTGCATGCCTGGTTGAGTTG | Start: 515 |
| Tm | 60.031 | 60.039 | End: 772 |
| Size | 20 | 20 | 258 |
| Primer 3 details |
|---|
| Sequence | GCATTGCGCAACAACGAATG | ATGGTCAGGCTGTTGCTCTC | Start: 1728 |
| Tm | 59.908 | 60.036 | End: 1938 |
| Size | 20 | 20 | 211 |
| Primer 4 details |
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| Sequence | GAGAGCAACAGCCTGACCAT | GGCATCCGATTTGCAGGTTC | Start: 1919 |
| Tm | 60.036 | 59.899 | End: 2077 |
| Size | 20 | 20 | 159 |
| Primer 5 details |
|---|
| Sequence | GGCAACAAAACCAGGCATGT | ATGGTCAGGCTGTTGCTCTC | Start: 1832 |
| Tm | 59.893 | 60.036 | End: 1938 |
| Size | 20 | 20 | 107 |
(AG)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCACCCCATCTGAGAGGTA | GCAACTGGCGTTACTTGCAA | Start: 515 |
| Tm | 60.031 | 59.97 | End: 712 |
| Size | 20 | 20 | 198 |
| Primer 2 details |
|---|
| Sequence | CCCACCCCATCTGAGAGGTA | GTGCATGCCTGGTTGAGTTG | Start: 515 |
| Tm | 60.031 | 60.039 | End: 772 |
| Size | 20 | 20 | 258 |
| Primer 3 details |
|---|
| Sequence | GCATTGCGCAACAACGAATG | ATGGTCAGGCTGTTGCTCTC | Start: 1728 |
| Tm | 59.908 | 60.036 | End: 1938 |
| Size | 20 | 20 | 211 |
| Primer 4 details |
|---|
| Sequence | GAGAGCAACAGCCTGACCAT | GGCATCCGATTTGCAGGTTC | Start: 1919 |
| Tm | 60.036 | 59.899 | End: 2077 |
| Size | 20 | 20 | 159 |
| Primer 5 details |
|---|
| Sequence | GGCAACAAAACCAGGCATGT | ATGGTCAGGCTGTTGCTCTC | Start: 1832 |
| Tm | 59.893 | 60.036 | End: 1938 |
| Size | 20 | 20 | 107 |
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