ssr_primer
Primer Details for TRINITY_DN12350_c0_g1_i9 |
|---|
| (TAT)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCGTGTTTTCGGTTCAAGCT | CCAAGTGGGACCCATCCTTC | Start: 666 |
| Tm | 59.971 | 60.034 | End: 780 |
| Size | 20 | 20 | 115 |
| Primer 2 details |
|---|
| Sequence | GAAGGATGGGTCCCACTTGG | TCGCAATCGGAAGGTGTTGA | Start: 761 |
| Tm | 60.034 | 59.966 | End: 1034 |
| Size | 20 | 20 | 274 |
| Primer 3 details |
|---|
| Sequence | GAAGGATGGGTCCCACTTGG | ATGTCGCAATCGGAAGGTGT | Start: 761 |
| Tm | 60.034 | 60.036 | End: 1037 |
| Size | 20 | 20 | 277 |
| Primer 4 details |
|---|
| Sequence | TCAACACCTTCCGATTGCGA | TTGCCAACCGTCGTCGATAA | Start: 1015 |
| Tm | 59.966 | 60.038 | End: 1193 |
| Size | 20 | 20 | 179 |
| Primer 5 details |
|---|
| Sequence | ACACCTTCCGATTGCGACAT | TTGCCAACCGTCGTCGATAA | Start: 1018 |
| Tm | 60.036 | 60.038 | End: 1193 |
| Size | 20 | 20 | 176 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCGTGTTTTCGGTTCAAGCT | CCAAGTGGGACCCATCCTTC | Start: 666 |
| Tm | 59.971 | 60.034 | End: 780 |
| Size | 20 | 20 | 115 |
| Primer 2 details |
|---|
| Sequence | GAAGGATGGGTCCCACTTGG | TCGCAATCGGAAGGTGTTGA | Start: 761 |
| Tm | 60.034 | 59.966 | End: 1034 |
| Size | 20 | 20 | 274 |
| Primer 3 details |
|---|
| Sequence | GAAGGATGGGTCCCACTTGG | ATGTCGCAATCGGAAGGTGT | Start: 761 |
| Tm | 60.034 | 60.036 | End: 1037 |
| Size | 20 | 20 | 277 |
| Primer 4 details |
|---|
| Sequence | TCAACACCTTCCGATTGCGA | TTGCCAACCGTCGTCGATAA | Start: 1015 |
| Tm | 59.966 | 60.038 | End: 1193 |
| Size | 20 | 20 | 179 |
| Primer 5 details |
|---|
| Sequence | ACACCTTCCGATTGCGACAT | TTGCCAACCGTCGTCGATAA | Start: 1018 |
| Tm | 60.036 | 60.038 | End: 1193 |
| Size | 20 | 20 | 176 |
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