Primer Details for TRINITY_DN12347_c3_g1_i1 |
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| (A)17: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAACTAAAAGAGAAAGAAAAGGAACA | TCTCTCCCTCCTTCCTCTCC | Start: 30 |
| Tm | 57.979 | 59.069 | End: 159 |
| Size | 27 | 20 | 130 |
| Primer 2 details |
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| Sequence | ACAACTAAAAGAGAAAGAAAAGGAACA | CTCTCCCTCCTTCCTCTCCT | Start: 30 |
| Tm | 57.979 | 59.066 | End: 158 |
| Size | 27 | 20 | 129 |
| Primer 3 details |
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| Sequence | ACAACTAAAAGAGAAAGAAAAGGAACA | TTCTCTCCCTCCTTCCTCTCC | Start: 30 |
| Tm | 57.979 | 59.71 | End: 160 |
| Size | 27 | 21 | 131 |
| Primer 4 details |
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| Sequence | ACAACTAAAAGAGAAAGAAAAGGAACA | TCTCTCCCTCCTTCCTCTCCT | Start: 30 |
| Tm | 57.979 | 60.562 | End: 159 |
| Size | 27 | 21 | 130 |
| Primer 5 details |
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| Sequence | CAACTAAAAGAGAAAGAAAAGGAACAA | TCTCTCCCTCCTTCCTCTCC | Start: 31 |
| Tm | 57.145 | 59.069 | End: 159 |
| Size | 27 | 20 | 129 |
