Primer Details for TRINITY_DN12333_c2_g1_i1 | |||
|---|---|---|---|
| (T)13: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAAATTCGATGGAAAACACATGA | TGTGGAAAATGGCTTCAGTGT | Start: 66 |
| Tm | 57.675 | 58.335 | End: 234 |
| Size | 24 | 21 | 169 |
| Primer 2 details | |||
| Sequence | TCAAATTCGATGGAAAACACATGA | TGTGGAAAATGGCTTCAGTGTT | Start: 66 |
| Tm | 57.675 | 58.97 | End: 234 |
| Size | 24 | 22 | 169 |
| Primer 3 details | |||
| Sequence | TCAAATTCGATGGAAAACACATGA | TTGTGGAAAATGGCTTCAGTGT | Start: 66 |
| Tm | 57.675 | 58.97 | End: 235 |
| Size | 24 | 22 | 170 |
| Primer 4 details | |||
| Sequence | TCAAATTCGATGGAAAACACATGAA | TGTGGAAAATGGCTTCAGTGT | Start: 66 |
| Tm | 58.257 | 58.335 | End: 234 |
| Size | 25 | 21 | 169 |
| Primer 5 details | |||
| Sequence | TTCGATGGAAAACACATGAATACA | TGTGGAAAATGGCTTCAGTGT | Start: 71 |
| Tm | 57.19 | 58.335 | End: 234 |
| Size | 24 | 21 | 164 |
