ssr_primer
Primer Details for TRINITY_DN12333_c0_g1_i4 |
|---|
| (TTC)7: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCAGGTCTGACGACGAGGA | CACATCAATCTTGGCAGCGG | Start: 489 |
| Tm | 60.035 | 59.9 | End: 635 |
| Size | 20 | 20 | 147 |
| Primer 2 details |
|---|
| Sequence | CCGCTGCCAAGATTGATGTG | TCCTGCTGAGTCTCGTACGA | Start: 616 |
| Tm | 59.9 | 60.037 | End: 843 |
| Size | 20 | 20 | 228 |
| Primer 3 details |
|---|
| Sequence | AGTGATGGAGAGGGTGTCGA | CACATCAATCTTGGCAGCGG | Start: 519 |
| Tm | 59.96 | 59.9 | End: 635 |
| Size | 20 | 20 | 117 |
| Primer 4 details |
|---|
| Sequence | TCGATCTCACGGACCTCCTT | GAGGTTGGGAATGATGGGGG | Start: 1 |
| Tm | 60.034 | 60.106 | End: 163 |
| Size | 20 | 20 | 163 |
| Primer 5 details |
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| Sequence | TTCGATCTCACGGACCTCCT | GAGGTTGGGAATGATGGGGG | Start: 0 |
| Tm | 60.034 | 60.106 | End: 163 |
| Size | 20 | 20 | 164 |
(T)11gttttcgttttccttttacatttgttgttaggtttgtttatgttttatttgccctctctaaccttcacagtctcggcattagatc(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCAGGTCTGACGACGAGGA | CACATCAATCTTGGCAGCGG | Start: 489 |
| Tm | 60.035 | 59.9 | End: 635 |
| Size | 20 | 20 | 147 |
| Primer 2 details |
|---|
| Sequence | CCGCTGCCAAGATTGATGTG | TCCTGCTGAGTCTCGTACGA | Start: 616 |
| Tm | 59.9 | 60.037 | End: 843 |
| Size | 20 | 20 | 228 |
| Primer 3 details |
|---|
| Sequence | AGTGATGGAGAGGGTGTCGA | CACATCAATCTTGGCAGCGG | Start: 519 |
| Tm | 59.96 | 59.9 | End: 635 |
| Size | 20 | 20 | 117 |
| Primer 4 details |
|---|
| Sequence | TCGATCTCACGGACCTCCTT | GAGGTTGGGAATGATGGGGG | Start: 1 |
| Tm | 60.034 | 60.106 | End: 163 |
| Size | 20 | 20 | 163 |
| Primer 5 details |
|---|
| Sequence | TTCGATCTCACGGACCTCCT | GAGGTTGGGAATGATGGGGG | Start: 0 |
| Tm | 60.034 | 60.106 | End: 163 |
| Size | 20 | 20 | 164 |
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