ssr_primer
Primer Details for TRINITY_DN12325_c0_g1_i1 |
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| (A)10ctcacataaaaaccatttagatgaataaaaaattctttctaattatattgatattagattttatttcccaattttgtgg(T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCATCCCCCACAAAGCAAT | GCTTGGCACCCATCCTTTTG | Start: 24 |
| Tm | 59.883 | 60.037 | End: 152 |
| Size | 20 | 20 | 129 |
| Primer 2 details |
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| Sequence | ACCATCCCCCACAAAGCAAT | AGCTTGGCACCCATCCTTTT | Start: 24 |
| Tm | 59.883 | 60.179 | End: 153 |
| Size | 20 | 20 | 130 |
| Primer 3 details |
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| Sequence | CAAAAGGATGGGTGCCAAGC | GCTCCGAGTTTGGCCTGTTA | Start: 133 |
| Tm | 60.037 | 60.321 | End: 387 |
| Size | 20 | 20 | 255 |
| Primer 4 details |
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| Sequence | CAAAAGGATGGGTGCCAAGC | TGGTTAATTGGAGGCGGTGG | Start: 133 |
| Tm | 60.037 | 60.323 | End: 303 |
| Size | 20 | 20 | 171 |
| Primer 5 details |
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| Sequence | TCCCCCACAAAGCAATAGCC | GCTTGGCACCCATCCTTTTG | Start: 28 |
| Tm | 60.324 | 60.037 | End: 152 |
| Size | 20 | 20 | 125 |
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