ssr_primer
Primer Details for TRINITY_DN12322_c1_g1_i2 |
|---|
| (T)20: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGCCAGAACTTCAGGACAC | ATGCCCACAGCTTCATTGGA | Start: 541 |
| Tm | 59.966 | 59.96 | End: 649 |
| Size | 20 | 20 | 109 |
| Primer 2 details |
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| Sequence | TCGCCAGAACTTCAGGACAC | TGAGCGTTGGTTGTTTGCTG | Start: 541 |
| Tm | 59.966 | 59.9 | End: 747 |
| Size | 20 | 20 | 207 |
| Primer 3 details |
|---|
| Sequence | TCCAATGAAGCTGTGGGCAT | TGAGCGTTGGTTGTTTGCTG | Start: 630 |
| Tm | 59.96 | 59.9 | End: 747 |
| Size | 20 | 20 | 118 |
| Primer 4 details |
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| Sequence | TACCATCGCCAATGCCCTTT | GTCGGTTGGGTCCACTCTTT | Start: 1982 |
| Tm | 60.034 | 59.892 | End: 2197 |
| Size | 20 | 20 | 216 |
| Primer 5 details |
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| Sequence | CTCCCCATCCTACTCCTGCT | CAATGTGTGTTCCAAGGCGG | Start: 2481 |
| Tm | 60.105 | 60.039 | End: 2640 |
| Size | 20 | 20 | 160 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGCCAGAACTTCAGGACAC | ATGCCCACAGCTTCATTGGA | Start: 541 |
| Tm | 59.966 | 59.96 | End: 649 |
| Size | 20 | 20 | 109 |
| Primer 2 details |
|---|
| Sequence | TCGCCAGAACTTCAGGACAC | TGAGCGTTGGTTGTTTGCTG | Start: 541 |
| Tm | 59.966 | 59.9 | End: 747 |
| Size | 20 | 20 | 207 |
| Primer 3 details |
|---|
| Sequence | TCCAATGAAGCTGTGGGCAT | TGAGCGTTGGTTGTTTGCTG | Start: 630 |
| Tm | 59.96 | 59.9 | End: 747 |
| Size | 20 | 20 | 118 |
| Primer 4 details |
|---|
| Sequence | TACCATCGCCAATGCCCTTT | GTCGGTTGGGTCCACTCTTT | Start: 1982 |
| Tm | 60.034 | 59.892 | End: 2197 |
| Size | 20 | 20 | 216 |
| Primer 5 details |
|---|
| Sequence | CTCCCCATCCTACTCCTGCT | CAATGTGTGTTCCAAGGCGG | Start: 2481 |
| Tm | 60.105 | 60.039 | End: 2640 |
| Size | 20 | 20 | 160 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGCCAGAACTTCAGGACAC | ATGCCCACAGCTTCATTGGA | Start: 541 |
| Tm | 59.966 | 59.96 | End: 649 |
| Size | 20 | 20 | 109 |
| Primer 2 details |
|---|
| Sequence | TCGCCAGAACTTCAGGACAC | TGAGCGTTGGTTGTTTGCTG | Start: 541 |
| Tm | 59.966 | 59.9 | End: 747 |
| Size | 20 | 20 | 207 |
| Primer 3 details |
|---|
| Sequence | TCCAATGAAGCTGTGGGCAT | TGAGCGTTGGTTGTTTGCTG | Start: 630 |
| Tm | 59.96 | 59.9 | End: 747 |
| Size | 20 | 20 | 118 |
| Primer 4 details |
|---|
| Sequence | TACCATCGCCAATGCCCTTT | GTCGGTTGGGTCCACTCTTT | Start: 1982 |
| Tm | 60.034 | 59.892 | End: 2197 |
| Size | 20 | 20 | 216 |
| Primer 5 details |
|---|
| Sequence | CTCCCCATCCTACTCCTGCT | CAATGTGTGTTCCAAGGCGG | Start: 2481 |
| Tm | 60.105 | 60.039 | End: 2640 |
| Size | 20 | 20 | 160 |
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