ssr_primer
Primer Details for TRINITY_DN12321_c1_g1_i1 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTAGCAACTTGTGGTTTGGGC | TGTCTTCAAAACTGCAGCCC | Start: 256 |
| Tm | 60 | 58.968 | End: 355 |
| Size | 21 | 20 | 100 |
| Primer 2 details |
|---|
| Sequence | AGGACGGCCATAAAGAGAAGT | CAAAACTGCAGCCCAAGTCG | Start: 201 |
| Tm | 58.811 | 60.318 | End: 349 |
| Size | 21 | 20 | 149 |
| Primer 3 details |
|---|
| Sequence | TCTGTGAGTGACATTATGGCGA | ATGCCCAAACCACAAGTTGC | Start: 122 |
| Tm | 59.504 | 59.893 | End: 278 |
| Size | 22 | 20 | 157 |
| Primer 4 details |
|---|
| Sequence | CTAGCAACTTGTGGTTTGGGC | ATGTCTTCAAAACTGCAGCCC | Start: 256 |
| Tm | 60 | 59.385 | End: 356 |
| Size | 21 | 21 | 101 |
| Primer 5 details |
|---|
| Sequence | TAGCAACTTGTGGTTTGGGC | ATGTCTTCAAAACTGCAGCCC | Start: 257 |
| Tm | 58.963 | 59.385 | End: 356 |
| Size | 20 | 21 | 100 |
(T)22: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTAGCAACTTGTGGTTTGGGC | TGTCTTCAAAACTGCAGCCC | Start: 256 |
| Tm | 60 | 58.968 | End: 355 |
| Size | 21 | 20 | 100 |
| Primer 2 details |
|---|
| Sequence | AGGACGGCCATAAAGAGAAGT | CAAAACTGCAGCCCAAGTCG | Start: 201 |
| Tm | 58.811 | 60.318 | End: 349 |
| Size | 21 | 20 | 149 |
| Primer 3 details |
|---|
| Sequence | TCTGTGAGTGACATTATGGCGA | ATGCCCAAACCACAAGTTGC | Start: 122 |
| Tm | 59.504 | 59.893 | End: 278 |
| Size | 22 | 20 | 157 |
| Primer 4 details |
|---|
| Sequence | CTAGCAACTTGTGGTTTGGGC | ATGTCTTCAAAACTGCAGCCC | Start: 256 |
| Tm | 60 | 59.385 | End: 356 |
| Size | 21 | 21 | 101 |
| Primer 5 details |
|---|
| Sequence | TAGCAACTTGTGGTTTGGGC | ATGTCTTCAAAACTGCAGCCC | Start: 257 |
| Tm | 58.963 | 59.385 | End: 356 |
| Size | 20 | 21 | 100 |
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