Primer Details for TRINITY_DN12318_c4_g1_i1 | |||
|---|---|---|---|
| (A)12gttaaaaaa(AAAC)5: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGGATTGCAAAACCTTTCCA | ACACCTCAAAAGAAAACTCCTGA | Start: 20 |
| Tm | 58.312 | 58.145 | End: 184 |
| Size | 21 | 23 | 165 |
| Primer 2 details | |||
| Sequence | AGGGATTGCAAAACCTTTCCA | TGAACACCTCAAAAGAAAACTCCTG | Start: 20 |
| Tm | 58.312 | 59.875 | End: 187 |
| Size | 21 | 25 | 168 |
| Primer 3 details | |||
| Sequence | AGGGATTGCAAAACCTTTCCAA | ACACCTCAAAAGAAAACTCCTGA | Start: 20 |
| Tm | 58.955 | 58.145 | End: 184 |
| Size | 22 | 23 | 165 |
| Primer 4 details | |||
| Sequence | AAGGGATTGCAAAACCTTTCCA | ACACCTCAAAAGAAAACTCCTGA | Start: 19 |
| Tm | 58.955 | 58.145 | End: 184 |
| Size | 22 | 23 | 166 |
| Primer 5 details | |||
| Sequence | AGGGATTGCAAAACCTTTCCA | ACACCTCAAAAGAAAACTCCTGAA | Start: 20 |
| Tm | 58.312 | 58.739 | End: 184 |
| Size | 21 | 24 | 165 |
