ssr_primer
Primer Details for TRINITY_DN12294_c1_g3_i4 |
|---|
| (A)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TAGGCCTACAGTCCCCAGAC | TGTCTTTTAAGCTGCGGGGT | Start: 1295 |
| Tm | 60.032 | 59.89 | End: 1402 |
| Size | 20 | 20 | 108 |
| Primer 2 details |
|---|
| Sequence | TAGGCCTACAGTCCCCAGAC | CACTCAGGGTGTGCTGTCAA | Start: 1295 |
| Tm | 60.032 | 60.179 | End: 1453 |
| Size | 20 | 20 | 159 |
| Primer 3 details |
|---|
| Sequence | CAGGGCCAACACTCTCAACA | AGCTCAGCTTTGCAAAAGCC | Start: 1675 |
| Tm | 60.179 | 59.967 | End: 1871 |
| Size | 20 | 20 | 197 |
| Primer 4 details |
|---|
| Sequence | CTGAGTGCACCACGGTGTAT | TGTTGAGAGTGTTGGCCCTG | Start: 1447 |
| Tm | 60.037 | 60.179 | End: 1694 |
| Size | 20 | 20 | 248 |
| Primer 5 details |
|---|
| Sequence | CACCCCTAACTTTGGCACCT | TGTCTTTTAAGCTGCGGGGT | Start: 1264 |
| Tm | 59.888 | 59.89 | End: 1402 |
| Size | 20 | 20 | 139 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TAGGCCTACAGTCCCCAGAC | TGTCTTTTAAGCTGCGGGGT | Start: 1295 |
| Tm | 60.032 | 59.89 | End: 1402 |
| Size | 20 | 20 | 108 |
| Primer 2 details |
|---|
| Sequence | TAGGCCTACAGTCCCCAGAC | CACTCAGGGTGTGCTGTCAA | Start: 1295 |
| Tm | 60.032 | 60.179 | End: 1453 |
| Size | 20 | 20 | 159 |
| Primer 3 details |
|---|
| Sequence | CAGGGCCAACACTCTCAACA | AGCTCAGCTTTGCAAAAGCC | Start: 1675 |
| Tm | 60.179 | 59.967 | End: 1871 |
| Size | 20 | 20 | 197 |
| Primer 4 details |
|---|
| Sequence | CTGAGTGCACCACGGTGTAT | TGTTGAGAGTGTTGGCCCTG | Start: 1447 |
| Tm | 60.037 | 60.179 | End: 1694 |
| Size | 20 | 20 | 248 |
| Primer 5 details |
|---|
| Sequence | CACCCCTAACTTTGGCACCT | TGTCTTTTAAGCTGCGGGGT | Start: 1264 |
| Tm | 59.888 | 59.89 | End: 1402 |
| Size | 20 | 20 | 139 |
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