ssr_primer
Primer Details for TRINITY_DN12294_c1_g1_i16 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGGGTGTGCAAAATCGCAG | GAGAACGGCGCCTGTGTATA | Start: 180 |
| Tm | 59.756 | 59.898 | End: 311 |
| Size | 20 | 20 | 132 |
| Primer 2 details |
|---|
| Sequence | ATGGGTGTGCAAAATCGCAG | CGGCGCCTGTGTATACTCTT | Start: 180 |
| Tm | 59.756 | 59.898 | End: 306 |
| Size | 20 | 20 | 127 |
| Primer 3 details |
|---|
| Sequence | TCTTCCTCACTGTTCCCCCA | GTCATGGCTGGAGGTTGGTT | Start: 4475 |
| Tm | 60.105 | 60.251 | End: 4677 |
| Size | 20 | 20 | 203 |
| Primer 4 details |
|---|
| Sequence | GGCCAATCCCTGATGGTCTC | GGTGGAGGAATTCGAGCCAT | Start: 2792 |
| Tm | 60.179 | 59.819 | End: 2943 |
| Size | 20 | 20 | 152 |
| Primer 5 details |
|---|
| Sequence | GGGTGTGCAAAATCGCAGAA | GAGAACGGCGCCTGTGTATA | Start: 182 |
| Tm | 59.686 | 59.898 | End: 311 |
| Size | 20 | 20 | 130 |
(A)13ggaaaaaagg(A)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGGGTGTGCAAAATCGCAG | GAGAACGGCGCCTGTGTATA | Start: 180 |
| Tm | 59.756 | 59.898 | End: 311 |
| Size | 20 | 20 | 132 |
| Primer 2 details |
|---|
| Sequence | ATGGGTGTGCAAAATCGCAG | CGGCGCCTGTGTATACTCTT | Start: 180 |
| Tm | 59.756 | 59.898 | End: 306 |
| Size | 20 | 20 | 127 |
| Primer 3 details |
|---|
| Sequence | TCTTCCTCACTGTTCCCCCA | GTCATGGCTGGAGGTTGGTT | Start: 4475 |
| Tm | 60.105 | 60.251 | End: 4677 |
| Size | 20 | 20 | 203 |
| Primer 4 details |
|---|
| Sequence | GGCCAATCCCTGATGGTCTC | GGTGGAGGAATTCGAGCCAT | Start: 2792 |
| Tm | 60.179 | 59.819 | End: 2943 |
| Size | 20 | 20 | 152 |
| Primer 5 details |
|---|
| Sequence | GGGTGTGCAAAATCGCAGAA | GAGAACGGCGCCTGTGTATA | Start: 182 |
| Tm | 59.686 | 59.898 | End: 311 |
| Size | 20 | 20 | 130 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGGGTGTGCAAAATCGCAG | GAGAACGGCGCCTGTGTATA | Start: 180 |
| Tm | 59.756 | 59.898 | End: 311 |
| Size | 20 | 20 | 132 |
| Primer 2 details |
|---|
| Sequence | ATGGGTGTGCAAAATCGCAG | CGGCGCCTGTGTATACTCTT | Start: 180 |
| Tm | 59.756 | 59.898 | End: 306 |
| Size | 20 | 20 | 127 |
| Primer 3 details |
|---|
| Sequence | TCTTCCTCACTGTTCCCCCA | GTCATGGCTGGAGGTTGGTT | Start: 4475 |
| Tm | 60.105 | 60.251 | End: 4677 |
| Size | 20 | 20 | 203 |
| Primer 4 details |
|---|
| Sequence | GGCCAATCCCTGATGGTCTC | GGTGGAGGAATTCGAGCCAT | Start: 2792 |
| Tm | 60.179 | 59.819 | End: 2943 |
| Size | 20 | 20 | 152 |
| Primer 5 details |
|---|
| Sequence | GGGTGTGCAAAATCGCAGAA | GAGAACGGCGCCTGTGTATA | Start: 182 |
| Tm | 59.686 | 59.898 | End: 311 |
| Size | 20 | 20 | 130 |
(AG)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGGGTGTGCAAAATCGCAG | GAGAACGGCGCCTGTGTATA | Start: 180 |
| Tm | 59.756 | 59.898 | End: 311 |
| Size | 20 | 20 | 132 |
| Primer 2 details |
|---|
| Sequence | ATGGGTGTGCAAAATCGCAG | CGGCGCCTGTGTATACTCTT | Start: 180 |
| Tm | 59.756 | 59.898 | End: 306 |
| Size | 20 | 20 | 127 |
| Primer 3 details |
|---|
| Sequence | TCTTCCTCACTGTTCCCCCA | GTCATGGCTGGAGGTTGGTT | Start: 4475 |
| Tm | 60.105 | 60.251 | End: 4677 |
| Size | 20 | 20 | 203 |
| Primer 4 details |
|---|
| Sequence | GGCCAATCCCTGATGGTCTC | GGTGGAGGAATTCGAGCCAT | Start: 2792 |
| Tm | 60.179 | 59.819 | End: 2943 |
| Size | 20 | 20 | 152 |
| Primer 5 details |
|---|
| Sequence | GGGTGTGCAAAATCGCAGAA | GAGAACGGCGCCTGTGTATA | Start: 182 |
| Tm | 59.686 | 59.898 | End: 311 |
| Size | 20 | 20 | 130 |
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