ssr_primer
Primer Details for TRINITY_DN12291_c2_g1_i5 |
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| (T)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATCCCCTCGATGCTTGCAA | GGTTGACAGAATGGGCTCCA | Start: 2380 |
| Tm | 60.034 | 59.962 | End: 2552 |
| Size | 20 | 20 | 173 |
| Primer 2 details |
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| Sequence | AATCCCCTCGATGCTTGCAA | TGGTTGACAGAATGGGCTCC | Start: 2380 |
| Tm | 60.034 | 59.962 | End: 2553 |
| Size | 20 | 20 | 174 |
| Primer 3 details |
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| Sequence | CCAATCCCAAGCTCCAGTGT | AGACGCAATTCCTTGTCCGT | Start: 3643 |
| Tm | 59.961 | 59.965 | End: 3863 |
| Size | 20 | 20 | 221 |
| Primer 4 details |
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| Sequence | CCAATCCCAAGCTCCAGTGT | CCATATGGATCTCACCGGCC | Start: 3643 |
| Tm | 59.961 | 60.036 | End: 3912 |
| Size | 20 | 20 | 270 |
| Primer 5 details |
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| Sequence | CAGTACAGCCCATGCAAGGA | AAGGGTTTAAGGAGCAGCCC | Start: 3235 |
| Tm | 60.035 | 59.96 | End: 3462 |
| Size | 20 | 20 | 228 |
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