ssr_primer
Primer Details for TRINITY_DN12291_c0_g1_i2 |
|---|
| (T)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCCTAGACCTTGACGGCA | TCGACGCACAAAACTCTCGA | Start: 335 |
| Tm | 59.961 | 59.97 | End: 543 |
| Size | 20 | 20 | 209 |
| Primer 2 details |
|---|
| Sequence | CCGTGCCCTTTACCCATGAT | TCGACGCACAAAACTCTCGA | Start: 297 |
| Tm | 60.107 | 59.97 | End: 543 |
| Size | 20 | 20 | 247 |
| Primer 3 details |
|---|
| Sequence | CTGTACCAGAAACGGCGGAT | TCTGTAATGCTGCTGCTGCT | Start: 2203 |
| Tm | 60.108 | 60.036 | End: 2305 |
| Size | 20 | 20 | 103 |
| Primer 4 details |
|---|
| Sequence | TCTCCTAGACCTTGACGGCA | GTCGACGCACAAAACTCTCG | Start: 335 |
| Tm | 59.961 | 59.841 | End: 544 |
| Size | 20 | 20 | 210 |
| Primer 5 details |
|---|
| Sequence | TCTCCTAGACCTTGACGGCA | CGTCGACGCACAAAACTCTC | Start: 335 |
| Tm | 59.961 | 59.841 | End: 545 |
| Size | 20 | 20 | 211 |
(A)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCCTAGACCTTGACGGCA | TCGACGCACAAAACTCTCGA | Start: 335 |
| Tm | 59.961 | 59.97 | End: 543 |
| Size | 20 | 20 | 209 |
| Primer 2 details |
|---|
| Sequence | CCGTGCCCTTTACCCATGAT | TCGACGCACAAAACTCTCGA | Start: 297 |
| Tm | 60.107 | 59.97 | End: 543 |
| Size | 20 | 20 | 247 |
| Primer 3 details |
|---|
| Sequence | CTGTACCAGAAACGGCGGAT | TCTGTAATGCTGCTGCTGCT | Start: 2203 |
| Tm | 60.108 | 60.036 | End: 2305 |
| Size | 20 | 20 | 103 |
| Primer 4 details |
|---|
| Sequence | TCTCCTAGACCTTGACGGCA | GTCGACGCACAAAACTCTCG | Start: 335 |
| Tm | 59.961 | 59.841 | End: 544 |
| Size | 20 | 20 | 210 |
| Primer 5 details |
|---|
| Sequence | TCTCCTAGACCTTGACGGCA | CGTCGACGCACAAAACTCTC | Start: 335 |
| Tm | 59.961 | 59.841 | End: 545 |
| Size | 20 | 20 | 211 |
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