ssr_primer
Primer Details for TRINITY_DN12290_c1_g4_i11 |
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| (AT)8: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGGCTCTTGGAGGGTATCT | CAACGTATGCAAGGGGGACT | Start: 1653 |
| Tm | 60.032 | 60.035 | End: 1849 |
| Size | 20 | 20 | 197 |
| Primer 2 details |
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| Sequence | ACGGCTCTTGGAGGGTATCT | TCAACGTATGCAAGGGGGAC | Start: 1653 |
| Tm | 60.032 | 60.036 | End: 1850 |
| Size | 20 | 20 | 198 |
| Primer 3 details |
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| Sequence | TGCGATGGTTGTTATGGGCT | GACATCGCAAACCTCTCCCA | Start: 469 |
| Tm | 60.035 | 60.036 | End: 668 |
| Size | 20 | 20 | 200 |
| Primer 4 details |
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| Sequence | CGAAGCTTGTCGTTCTCCCT | CAACGTATGCAAGGGGGACT | Start: 1592 |
| Tm | 60.038 | 60.035 | End: 1849 |
| Size | 20 | 20 | 258 |
| Primer 5 details |
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| Sequence | TCGAAGCTTGTCGTTCTCCC | CAACGTATGCAAGGGGGACT | Start: 1591 |
| Tm | 60.039 | 60.035 | End: 1849 |
| Size | 20 | 20 | 259 |
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