ssr_primer
Primer Details for TRINITY_DN12288_c1_g5_i1 |
|---|
| (T)25: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGAATGGTTTGGGGCAACG | TGGAGCTGCTTCATCACCTC | Start: 145 |
| Tm | 59.756 | 59.749 | End: 383 |
| Size | 20 | 20 | 239 |
| Primer 2 details |
|---|
| Sequence | GGTTTGGGGCAACGTAGCTA | TGGAGCTGCTTCATCACCTC | Start: 151 |
| Tm | 60.322 | 59.749 | End: 383 |
| Size | 20 | 20 | 233 |
| Primer 3 details |
|---|
| Sequence | TACTCATGTTGGACGGCGTG | TGGAGCTGCTTCATCACCTC | Start: 223 |
| Tm | 60.389 | 59.749 | End: 383 |
| Size | 20 | 20 | 161 |
| Primer 4 details |
|---|
| Sequence | AGAATGGTTTGGGGCAACGT | TGGAGCTGCTTCATCACCTC | Start: 146 |
| Tm | 60.469 | 59.749 | End: 383 |
| Size | 20 | 20 | 238 |
| Primer 5 details |
|---|
| Sequence | GAGAATGGTTTGGGGCAACG | GGAGCTGCTTCATCACCTCT | Start: 145 |
| Tm | 59.756 | 59.459 | End: 382 |
| Size | 20 | 20 | 238 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGAATGGTTTGGGGCAACG | TGGAGCTGCTTCATCACCTC | Start: 145 |
| Tm | 59.756 | 59.749 | End: 383 |
| Size | 20 | 20 | 239 |
| Primer 2 details |
|---|
| Sequence | GGTTTGGGGCAACGTAGCTA | TGGAGCTGCTTCATCACCTC | Start: 151 |
| Tm | 60.322 | 59.749 | End: 383 |
| Size | 20 | 20 | 233 |
| Primer 3 details |
|---|
| Sequence | TACTCATGTTGGACGGCGTG | TGGAGCTGCTTCATCACCTC | Start: 223 |
| Tm | 60.389 | 59.749 | End: 383 |
| Size | 20 | 20 | 161 |
| Primer 4 details |
|---|
| Sequence | AGAATGGTTTGGGGCAACGT | TGGAGCTGCTTCATCACCTC | Start: 146 |
| Tm | 60.469 | 59.749 | End: 383 |
| Size | 20 | 20 | 238 |
| Primer 5 details |
|---|
| Sequence | GAGAATGGTTTGGGGCAACG | GGAGCTGCTTCATCACCTCT | Start: 145 |
| Tm | 59.756 | 59.459 | End: 382 |
| Size | 20 | 20 | 238 |
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