ssr_primer
Primer Details for TRINITY_DN12287_c4_g4_i1 |
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| (T)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCTTGCTCTTTGCCAGCGA | TCCCTGGAGACAAAGACCCT | Start: 276 |
| Tm | 60.322 | 59.806 | End: 376 |
| Size | 20 | 20 | 101 |
| Primer 2 details |
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| Sequence | AATCTTGCTCTTTGCCAGCG | TCCCTGGAGACAAAGACCCT | Start: 275 |
| Tm | 59.475 | 59.806 | End: 376 |
| Size | 20 | 20 | 102 |
| Primer 3 details |
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| Sequence | TCTTGCTCTTTGCCAGCGAA | TCCCTGGAGACAAAGACCCT | Start: 277 |
| Tm | 60.535 | 59.806 | End: 376 |
| Size | 20 | 20 | 100 |
| Primer 4 details |
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| Sequence | AGGGTCTTTGTCTCCAGGGA | CACAGCTACCTTCCAGCTCA | Start: 357 |
| Tm | 59.806 | 59.39 | End: 552 |
| Size | 20 | 20 | 196 |
| Primer 5 details |
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| Sequence | TTGCTCTTTGCCAGCGAAAG | CACAGCTACCTTCCAGCTCA | Start: 279 |
| Tm | 59.688 | 59.39 | End: 552 |
| Size | 20 | 20 | 274 |
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