Primer Details for TRINITY_DN12287_c10_g1_i1 | |||
|---|---|---|---|
| (T)15: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGACAACAATTTTCTTGCGTATTGC | TGAAATAAAAGAACGGGCTTTTGC | Start: 85 |
| Tm | 59.825 | 59.252 | End: 279 |
| Size | 25 | 24 | 195 |
| Primer 2 details | |||
| Sequence | ACAACAATTTTCTTGCGTATTGC | TGAAATAAAAGAACGGGCTTTTGC | Start: 87 |
| Tm | 57.425 | 59.252 | End: 279 |
| Size | 23 | 24 | 193 |
| Primer 3 details | |||
| Sequence | GACAACAATTTTCTTGCGTATTGC | TGAAATAAAAGAACGGGCTTTTGC | Start: 86 |
| Tm | 58.401 | 59.252 | End: 279 |
| Size | 24 | 24 | 194 |
| Primer 4 details | |||
| Sequence | TGACTACTAGAGGATCATCACCT | TGAAATAAAAGAACGGGCTTTTGC | Start: 144 |
| Tm | 57.345 | 59.252 | End: 279 |
| Size | 23 | 24 | 136 |
| Primer 5 details | |||
| Sequence | TGACAACAATTTTCTTGCGTATTGC | GAAATAAAAGAACGGGCTTTTGC | Start: 85 |
| Tm | 59.825 | 57.736 | End: 278 |
| Size | 25 | 23 | 194 |
