ssr_primer
Primer Details for TRINITY_DN12280_c2_g1_i3 |
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| (A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCGATGTGTTAATGCCGTC | CGTTCTGAAACTGCGTTGCA | Start: 1147 |
| Tm | 59.972 | 59.973 | End: 1393 |
| Size | 20 | 20 | 247 |
| Primer 2 details |
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| Sequence | AGCGAGCGAAAATGGAAGGA | CGTTCTGAAACTGCGTTGCA | Start: 1291 |
| Tm | 60.036 | 59.973 | End: 1393 |
| Size | 20 | 20 | 103 |
| Primer 3 details |
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| Sequence | GGCGATGTGTTAATGCCGTC | TCCTTCCATTTTCGCTCGCT | Start: 1147 |
| Tm | 59.972 | 60.036 | End: 1310 |
| Size | 20 | 20 | 164 |
| Primer 4 details |
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| Sequence | TCGATCCATGGGACCTTCCT | TCCAACCGGTTTAGGATGCC | Start: 514 |
| Tm | 60.031 | 60.035 | End: 789 |
| Size | 20 | 20 | 276 |
| Primer 5 details |
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| Sequence | TCGATCCATGGGACCTTCCT | CCGTGTAAGGCCAGTTCTGT | Start: 514 |
| Tm | 60.031 | 59.965 | End: 653 |
| Size | 20 | 20 | 140 |
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