ssr_primer
Primer Details for TRINITY_DN12277_c5_g1_i7 |
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| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTTCCTTCTTCCGGCAGTT | GAGTTGCCTCCCTTCAGCAT | Start: 1131 |
| Tm | 59.963 | 60.035 | End: 1296 |
| Size | 20 | 20 | 166 |
| Primer 2 details |
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| Sequence | TTTCCCTTCCTTCTTCCGGC | GAGTTGCCTCCCTTCAGCAT | Start: 1127 |
| Tm | 59.962 | 60.035 | End: 1296 |
| Size | 20 | 20 | 170 |
| Primer 3 details |
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| Sequence | ACAGGAGAGAGCTATGGGGG | TCAAACCGGTGGACCACAAA | Start: 652 |
| Tm | 60.105 | 60.034 | End: 829 |
| Size | 20 | 20 | 178 |
| Primer 4 details |
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| Sequence | TTGCTTATGGCGAGGCAGAA | CCCCCATAGCTCTCTCCTGT | Start: 392 |
| Tm | 60.036 | 60.105 | End: 671 |
| Size | 20 | 20 | 280 |
| Primer 5 details |
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| Sequence | ACAGGAGAGAGCTATGGGGG | AGATCAAACCGGTGGACCAC | Start: 652 |
| Tm | 60.105 | 59.964 | End: 832 |
| Size | 20 | 20 | 181 |
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