ssr_primer
Primer Details for TRINITY_DN12275_c4_g1_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGGTGGGGGAATCTCTCAA | CTGTGAACGAAGCCGACCTA | Start: 238 |
| Tm | 59.879 | 59.757 | End: 394 |
| Size | 20 | 20 | 157 |
| Primer 2 details |
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| Sequence | ATGCTTCTTCATGCCGGTCT | CTGTGAACGAAGCCGACCTA | Start: 275 |
| Tm | 59.746 | 59.757 | End: 394 |
| Size | 20 | 20 | 120 |
| Primer 3 details |
|---|
| Sequence | AGGGTGGGGGAATCTCTCAA | ATCTGTGAACGAAGCCGACC | Start: 238 |
| Tm | 59.879 | 60.39 | End: 396 |
| Size | 20 | 20 | 159 |
| Primer 4 details |
|---|
| Sequence | ATGCTTCTTCATGCCGGTCT | ATCTGTGAACGAAGCCGACC | Start: 275 |
| Tm | 59.746 | 60.39 | End: 396 |
| Size | 20 | 20 | 122 |
| Primer 5 details |
|---|
| Sequence | AGGGTGGGGGAATCTCTCAA | TGTGAACGAAGCCGACCTAA | Start: 238 |
| Tm | 59.879 | 59.325 | End: 393 |
| Size | 20 | 20 | 156 |
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