ssr_primer
Primer Details for TRINITY_DN12274_c2_g4_i1 |
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| (A)11tctctgaatgaaaccaaac(TCT)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAGATTCGGACTCTGCTCCC | GGCTCAAAGGGGGAGGAATC | Start: 321 |
| Tm | 59.896 | 60.106 | End: 474 |
| Size | 20 | 20 | 154 |
| Primer 2 details |
|---|
| Sequence | CCAGATTCGGACTCTGCTCC | GGCTCAAAGGGGGAGGAATC | Start: 320 |
| Tm | 59.896 | 60.106 | End: 474 |
| Size | 20 | 20 | 155 |
| Primer 3 details |
|---|
| Sequence | CCCAGATTCGGACTCTGCTC | GGCTCAAAGGGGGAGGAATC | Start: 319 |
| Tm | 59.896 | 60.106 | End: 474 |
| Size | 20 | 20 | 156 |
| Primer 4 details |
|---|
| Sequence | CAGATTCGGACTCTGCTCCC | AAAAGGCTCAAAGGGGGAGG | Start: 321 |
| Tm | 59.896 | 59.885 | End: 478 |
| Size | 20 | 20 | 158 |
| Primer 5 details |
|---|
| Sequence | CCAGATTCGGACTCTGCTCC | AAAAGGCTCAAAGGGGGAGG | Start: 320 |
| Tm | 59.896 | 59.885 | End: 478 |
| Size | 20 | 20 | 159 |
(T)15: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAGATTCGGACTCTGCTCCC | GGCTCAAAGGGGGAGGAATC | Start: 321 |
| Tm | 59.896 | 60.106 | End: 474 |
| Size | 20 | 20 | 154 |
| Primer 2 details |
|---|
| Sequence | CCAGATTCGGACTCTGCTCC | GGCTCAAAGGGGGAGGAATC | Start: 320 |
| Tm | 59.896 | 60.106 | End: 474 |
| Size | 20 | 20 | 155 |
| Primer 3 details |
|---|
| Sequence | CCCAGATTCGGACTCTGCTC | GGCTCAAAGGGGGAGGAATC | Start: 319 |
| Tm | 59.896 | 60.106 | End: 474 |
| Size | 20 | 20 | 156 |
| Primer 4 details |
|---|
| Sequence | CAGATTCGGACTCTGCTCCC | AAAAGGCTCAAAGGGGGAGG | Start: 321 |
| Tm | 59.896 | 59.885 | End: 478 |
| Size | 20 | 20 | 158 |
| Primer 5 details |
|---|
| Sequence | CCAGATTCGGACTCTGCTCC | AAAAGGCTCAAAGGGGGAGG | Start: 320 |
| Tm | 59.896 | 59.885 | End: 478 |
| Size | 20 | 20 | 159 |
(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAGATTCGGACTCTGCTCCC | GGCTCAAAGGGGGAGGAATC | Start: 321 |
| Tm | 59.896 | 60.106 | End: 474 |
| Size | 20 | 20 | 154 |
| Primer 2 details |
|---|
| Sequence | CCAGATTCGGACTCTGCTCC | GGCTCAAAGGGGGAGGAATC | Start: 320 |
| Tm | 59.896 | 60.106 | End: 474 |
| Size | 20 | 20 | 155 |
| Primer 3 details |
|---|
| Sequence | CCCAGATTCGGACTCTGCTC | GGCTCAAAGGGGGAGGAATC | Start: 319 |
| Tm | 59.896 | 60.106 | End: 474 |
| Size | 20 | 20 | 156 |
| Primer 4 details |
|---|
| Sequence | CAGATTCGGACTCTGCTCCC | AAAAGGCTCAAAGGGGGAGG | Start: 321 |
| Tm | 59.896 | 59.885 | End: 478 |
| Size | 20 | 20 | 158 |
| Primer 5 details |
|---|
| Sequence | CCAGATTCGGACTCTGCTCC | AAAAGGCTCAAAGGGGGAGG | Start: 320 |
| Tm | 59.896 | 59.885 | End: 478 |
| Size | 20 | 20 | 159 |
(AGA)6ggatgaagacga(AGG)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAGATTCGGACTCTGCTCCC | GGCTCAAAGGGGGAGGAATC | Start: 321 |
| Tm | 59.896 | 60.106 | End: 474 |
| Size | 20 | 20 | 154 |
| Primer 2 details |
|---|
| Sequence | CCAGATTCGGACTCTGCTCC | GGCTCAAAGGGGGAGGAATC | Start: 320 |
| Tm | 59.896 | 60.106 | End: 474 |
| Size | 20 | 20 | 155 |
| Primer 3 details |
|---|
| Sequence | CCCAGATTCGGACTCTGCTC | GGCTCAAAGGGGGAGGAATC | Start: 319 |
| Tm | 59.896 | 60.106 | End: 474 |
| Size | 20 | 20 | 156 |
| Primer 4 details |
|---|
| Sequence | CAGATTCGGACTCTGCTCCC | AAAAGGCTCAAAGGGGGAGG | Start: 321 |
| Tm | 59.896 | 59.885 | End: 478 |
| Size | 20 | 20 | 158 |
| Primer 5 details |
|---|
| Sequence | CCAGATTCGGACTCTGCTCC | AAAAGGCTCAAAGGGGGAGG | Start: 320 |
| Tm | 59.896 | 59.885 | End: 478 |
| Size | 20 | 20 | 159 |
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