ssr_primer
Primer Details for TRINITY_DN12274_c1_g1_i3 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGGAACTCGACAGCATGGA | GCCCCTGGAACTCTCTATGC | Start: 909 |
| Tm | 60.037 | 59.892 | End: 1135 |
| Size | 20 | 20 | 227 |
| Primer 2 details |
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| Sequence | GCATAGAGAGTTCCAGGGGC | TGAGTGGCAAGTCAATCCCC | Start: 1116 |
| Tm | 59.892 | 59.962 | End: 1217 |
| Size | 20 | 20 | 102 |
| Primer 3 details |
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| Sequence | GGATAACCCAACCCCCAAGG | TGGTAAGGCCTGGCATTTGT | Start: 430 |
| Tm | 60.033 | 59.886 | End: 531 |
| Size | 20 | 20 | 102 |
| Primer 4 details |
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| Sequence | ATTATCGTCAGGGCGTTGCT | TCCATGCTGTCGAGTTCCAC | Start: 725 |
| Tm | 59.822 | 60.037 | End: 928 |
| Size | 20 | 20 | 204 |
| Primer 5 details |
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| Sequence | CAGGGCGTTGCTGCAAATAG | TCCATGCTGTCGAGTTCCAC | Start: 733 |
| Tm | 60.179 | 60.037 | End: 928 |
| Size | 20 | 20 | 196 |
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