ssr_primer
Primer Details for TRINITY_DN12271_c0_g6_i2 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TAGATTCTACCCGGTGGCCA | CGCTTAAGCCTGAGGGGAAA | Start: 3235 |
| Tm | 60.032 | 60.035 | End: 3394 |
| Size | 20 | 20 | 160 |
| Primer 2 details |
|---|
| Sequence | GCAGTCGTTGTTGCGTTCTT | TCCGGAGCCTGAGGACTAAA | Start: 1141 |
| Tm | 59.972 | 59.959 | End: 1265 |
| Size | 20 | 20 | 125 |
| Primer 3 details |
|---|
| Sequence | GCAGTCGTTGTTGCGTTCTT | TTCCGGAGCCTGAGGACTAA | Start: 1141 |
| Tm | 59.972 | 59.959 | End: 1266 |
| Size | 20 | 20 | 126 |
| Primer 4 details |
|---|
| Sequence | TGTTCGTCGTTACATGGGCA | CGCTTAAGCCTGAGGGGAAA | Start: 3194 |
| Tm | 59.966 | 60.035 | End: 3394 |
| Size | 20 | 20 | 201 |
| Primer 5 details |
|---|
| Sequence | AATCCCAAGCCCAATCCCTG | GGCTGTGCAAGGGTGAAATG | Start: 3991 |
| Tm | 60.032 | 60.038 | End: 4101 |
| Size | 20 | 20 | 111 |
(CAG)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TAGATTCTACCCGGTGGCCA | CGCTTAAGCCTGAGGGGAAA | Start: 3235 |
| Tm | 60.032 | 60.035 | End: 3394 |
| Size | 20 | 20 | 160 |
| Primer 2 details |
|---|
| Sequence | GCAGTCGTTGTTGCGTTCTT | TCCGGAGCCTGAGGACTAAA | Start: 1141 |
| Tm | 59.972 | 59.959 | End: 1265 |
| Size | 20 | 20 | 125 |
| Primer 3 details |
|---|
| Sequence | GCAGTCGTTGTTGCGTTCTT | TTCCGGAGCCTGAGGACTAA | Start: 1141 |
| Tm | 59.972 | 59.959 | End: 1266 |
| Size | 20 | 20 | 126 |
| Primer 4 details |
|---|
| Sequence | TGTTCGTCGTTACATGGGCA | CGCTTAAGCCTGAGGGGAAA | Start: 3194 |
| Tm | 59.966 | 60.035 | End: 3394 |
| Size | 20 | 20 | 201 |
| Primer 5 details |
|---|
| Sequence | AATCCCAAGCCCAATCCCTG | GGCTGTGCAAGGGTGAAATG | Start: 3991 |
| Tm | 60.032 | 60.038 | End: 4101 |
| Size | 20 | 20 | 111 |
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