ssr_primer
Primer Details for TRINITY_DN12271_c0_g5_i1 |
|---|
| (T)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGGAGCGCAGTGATTCTGT | ATCGGAAGTGGGTGATTCGG | Start: 492 |
| Tm | 59.965 | 59.822 | End: 648 |
| Size | 20 | 20 | 157 |
| Primer 2 details |
|---|
| Sequence | TGTTGGAGCGCAGTGATTCT | ATCGGAAGTGGGTGATTCGG | Start: 490 |
| Tm | 59.965 | 59.822 | End: 648 |
| Size | 20 | 20 | 159 |
| Primer 3 details |
|---|
| Sequence | CCGAATCACCCACTTCCGAT | TTTCGGAATGAACTCGGGGG | Start: 629 |
| Tm | 59.822 | 60.036 | End: 888 |
| Size | 20 | 20 | 260 |
| Primer 4 details |
|---|
| Sequence | TCTCTGCTGCTGGTGTTGTT | AGAATCACTGCGCTCCAACA | Start: 339 |
| Tm | 59.819 | 59.965 | End: 509 |
| Size | 20 | 20 | 171 |
| Primer 5 details |
|---|
| Sequence | TTCTCTGCTGCTGGTGTTGT | AGAATCACTGCGCTCCAACA | Start: 338 |
| Tm | 59.819 | 59.965 | End: 509 |
| Size | 20 | 20 | 172 |
(TGT)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGGAGCGCAGTGATTCTGT | ATCGGAAGTGGGTGATTCGG | Start: 492 |
| Tm | 59.965 | 59.822 | End: 648 |
| Size | 20 | 20 | 157 |
| Primer 2 details |
|---|
| Sequence | TGTTGGAGCGCAGTGATTCT | ATCGGAAGTGGGTGATTCGG | Start: 490 |
| Tm | 59.965 | 59.822 | End: 648 |
| Size | 20 | 20 | 159 |
| Primer 3 details |
|---|
| Sequence | CCGAATCACCCACTTCCGAT | TTTCGGAATGAACTCGGGGG | Start: 629 |
| Tm | 59.822 | 60.036 | End: 888 |
| Size | 20 | 20 | 260 |
| Primer 4 details |
|---|
| Sequence | TCTCTGCTGCTGGTGTTGTT | AGAATCACTGCGCTCCAACA | Start: 339 |
| Tm | 59.819 | 59.965 | End: 509 |
| Size | 20 | 20 | 171 |
| Primer 5 details |
|---|
| Sequence | TTCTCTGCTGCTGGTGTTGT | AGAATCACTGCGCTCCAACA | Start: 338 |
| Tm | 59.819 | 59.965 | End: 509 |
| Size | 20 | 20 | 172 |
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