ssr_primer
Primer Details for TRINITY_DN12268_c2_g3_i6 |
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| (CCA)8cacaacacccttctgttttcctctgtttccccctttatataaatgagagaa(AG)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAACCCCATGCGAAAACAGC | AAGCTGTCGGCCTTAGCAAT | Start: 3436 |
| Tm | 59.967 | 60.035 | End: 3629 |
| Size | 20 | 20 | 194 |
| Primer 2 details |
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| Sequence | AGTTCCAGGCTCAGCGTAAC | ACACGGAGATTGTTGGGGAC | Start: 3051 |
| Tm | 60.037 | 59.964 | End: 3178 |
| Size | 20 | 20 | 128 |
| Primer 3 details |
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| Sequence | AGTTCCAGGCTCAGCGTAAC | TGGCGAGAGAAGACGAACAC | Start: 3051 |
| Tm | 60.037 | 60.039 | End: 3194 |
| Size | 20 | 20 | 144 |
| Primer 4 details |
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| Sequence | TGCTCGCCTCAATCTCCATC | GCTGTTTTCGCATGGGGTTT | Start: 3251 |
| Tm | 59.894 | 59.967 | End: 3455 |
| Size | 20 | 20 | 205 |
| Primer 5 details |
|---|
| Sequence | CCACCACACAACACCCTTCT | GCTGTTTTCGCATGGGGTTT | Start: 3290 |
| Tm | 60.107 | 59.967 | End: 3455 |
| Size | 20 | 20 | 166 |
(AAG)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAACCCCATGCGAAAACAGC | AAGCTGTCGGCCTTAGCAAT | Start: 3436 |
| Tm | 59.967 | 60.035 | End: 3629 |
| Size | 20 | 20 | 194 |
| Primer 2 details |
|---|
| Sequence | AGTTCCAGGCTCAGCGTAAC | ACACGGAGATTGTTGGGGAC | Start: 3051 |
| Tm | 60.037 | 59.964 | End: 3178 |
| Size | 20 | 20 | 128 |
| Primer 3 details |
|---|
| Sequence | AGTTCCAGGCTCAGCGTAAC | TGGCGAGAGAAGACGAACAC | Start: 3051 |
| Tm | 60.037 | 60.039 | End: 3194 |
| Size | 20 | 20 | 144 |
| Primer 4 details |
|---|
| Sequence | TGCTCGCCTCAATCTCCATC | GCTGTTTTCGCATGGGGTTT | Start: 3251 |
| Tm | 59.894 | 59.967 | End: 3455 |
| Size | 20 | 20 | 205 |
| Primer 5 details |
|---|
| Sequence | CCACCACACAACACCCTTCT | GCTGTTTTCGCATGGGGTTT | Start: 3290 |
| Tm | 60.107 | 59.967 | End: 3455 |
| Size | 20 | 20 | 166 |
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