ssr_primer
Primer Details for TRINITY_DN12268_c1_g1_i1 |
|---|
| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTCTCTCCCCACCGAAAAC | GGTGGAGGAAGACTTGGACC | Start: 939 |
| Tm | 60.036 | 59.675 | End: 1153 |
| Size | 20 | 20 | 215 |
| Primer 2 details |
|---|
| Sequence | CCAAAGAAACCGGCCTCTCT | GGTGGAGGAAGACTTGGACC | Start: 985 |
| Tm | 59.963 | 59.675 | End: 1153 |
| Size | 20 | 20 | 169 |
| Primer 3 details |
|---|
| Sequence | TTAGTCGCTCCTCTCTCCCC | GGTGGAGGAAGACTTGGACC | Start: 930 |
| Tm | 60.107 | 59.675 | End: 1153 |
| Size | 20 | 20 | 224 |
| Primer 4 details |
|---|
| Sequence | TCCTCTCTCCCCACCGAAAA | GGTGGAGGAAGACTTGGACC | Start: 938 |
| Tm | 60.179 | 59.675 | End: 1153 |
| Size | 20 | 20 | 216 |
| Primer 5 details |
|---|
| Sequence | GCACTCACACTACCTTGCCA | GGTGGAGGAAGACTTGGACC | Start: 968 |
| Tm | 60.25 | 59.675 | End: 1153 |
| Size | 20 | 20 | 186 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTCTCTCCCCACCGAAAAC | GGTGGAGGAAGACTTGGACC | Start: 939 |
| Tm | 60.036 | 59.675 | End: 1153 |
| Size | 20 | 20 | 215 |
| Primer 2 details |
|---|
| Sequence | CCAAAGAAACCGGCCTCTCT | GGTGGAGGAAGACTTGGACC | Start: 985 |
| Tm | 59.963 | 59.675 | End: 1153 |
| Size | 20 | 20 | 169 |
| Primer 3 details |
|---|
| Sequence | TTAGTCGCTCCTCTCTCCCC | GGTGGAGGAAGACTTGGACC | Start: 930 |
| Tm | 60.107 | 59.675 | End: 1153 |
| Size | 20 | 20 | 224 |
| Primer 4 details |
|---|
| Sequence | TCCTCTCTCCCCACCGAAAA | GGTGGAGGAAGACTTGGACC | Start: 938 |
| Tm | 60.179 | 59.675 | End: 1153 |
| Size | 20 | 20 | 216 |
| Primer 5 details |
|---|
| Sequence | GCACTCACACTACCTTGCCA | GGTGGAGGAAGACTTGGACC | Start: 968 |
| Tm | 60.25 | 59.675 | End: 1153 |
| Size | 20 | 20 | 186 |
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