ssr_primer
Primer Details for TRINITY_DN12268_c0_g1_i3 |
|---|
| (T)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCACCTTCCCTCCCTCTGTT | CCCTCGCACAGTTCTGCTAA | Start: 2213 |
| Tm | 59.806 | 60.037 | End: 2341 |
| Size | 20 | 20 | 129 |
| Primer 2 details |
|---|
| Sequence | TTCACCTTCCCTCCCTCTGT | CCCTCGCACAGTTCTGCTAA | Start: 2212 |
| Tm | 59.806 | 60.037 | End: 2341 |
| Size | 20 | 20 | 130 |
| Primer 3 details |
|---|
| Sequence | ACTGGATCGAGTGGGCAATG | GATTTGTTCGACCCAGCAGC | Start: 498 |
| Tm | 60.108 | 59.831 | End: 694 |
| Size | 20 | 20 | 197 |
| Primer 4 details |
|---|
| Sequence | ACTGGATCGAGTGGGCAATG | GCAGATTTGTTCGACCCAGC | Start: 498 |
| Tm | 60.108 | 59.831 | End: 697 |
| Size | 20 | 20 | 200 |
| Primer 5 details |
|---|
| Sequence | AGGAAGAAGGCACCTAACGC | AACAGCGATTGGGGCTTCTT | Start: 170 |
| Tm | 60.036 | 60.251 | End: 329 |
| Size | 20 | 20 | 160 |
(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCACCTTCCCTCCCTCTGTT | CCCTCGCACAGTTCTGCTAA | Start: 2213 |
| Tm | 59.806 | 60.037 | End: 2341 |
| Size | 20 | 20 | 129 |
| Primer 2 details |
|---|
| Sequence | TTCACCTTCCCTCCCTCTGT | CCCTCGCACAGTTCTGCTAA | Start: 2212 |
| Tm | 59.806 | 60.037 | End: 2341 |
| Size | 20 | 20 | 130 |
| Primer 3 details |
|---|
| Sequence | ACTGGATCGAGTGGGCAATG | GATTTGTTCGACCCAGCAGC | Start: 498 |
| Tm | 60.108 | 59.831 | End: 694 |
| Size | 20 | 20 | 197 |
| Primer 4 details |
|---|
| Sequence | ACTGGATCGAGTGGGCAATG | GCAGATTTGTTCGACCCAGC | Start: 498 |
| Tm | 60.108 | 59.831 | End: 697 |
| Size | 20 | 20 | 200 |
| Primer 5 details |
|---|
| Sequence | AGGAAGAAGGCACCTAACGC | AACAGCGATTGGGGCTTCTT | Start: 170 |
| Tm | 60.036 | 60.251 | End: 329 |
| Size | 20 | 20 | 160 |
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