ssr_primer
Primer Details for TRINITY_DN12267_c0_g1_i1 |
|---|
| (A)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCTTCAGCTGGATCCTCGA | TAGAGAGGGGAGCGGGTATG | Start: 641 |
| Tm | 60.033 | 59.887 | End: 756 |
| Size | 20 | 20 | 116 |
| Primer 2 details |
|---|
| Sequence | TCCTTCAGCTGGATCCTCGA | GTAGAGAGGGGAGCGGGTAT | Start: 641 |
| Tm | 60.033 | 59.887 | End: 757 |
| Size | 20 | 20 | 117 |
| Primer 3 details |
|---|
| Sequence | CATACCCGCTCCCCTCTCTA | CGAAGGCTCGTGAAGTCCTT | Start: 737 |
| Tm | 59.887 | 60.038 | End: 990 |
| Size | 20 | 20 | 254 |
| Primer 4 details |
|---|
| Sequence | ATACCCGCTCCCCTCTCTAC | CGAAGGCTCGTGAAGTCCTT | Start: 738 |
| Tm | 59.887 | 60.038 | End: 990 |
| Size | 20 | 20 | 253 |
| Primer 5 details |
|---|
| Sequence | GCTAACCGAAAGTTGCGACC | TCGAGGATCCAGCTGAAGGA | Start: 438 |
| Tm | 59.835 | 60.033 | End: 660 |
| Size | 20 | 20 | 223 |
(A)10caataaaaccggatccataaaataagatcaaatacgaatctgaacaaaatgaaacgacaaaaattgcacatcaagacaggaagtgaagtaacg(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCTTCAGCTGGATCCTCGA | TAGAGAGGGGAGCGGGTATG | Start: 641 |
| Tm | 60.033 | 59.887 | End: 756 |
| Size | 20 | 20 | 116 |
| Primer 2 details |
|---|
| Sequence | TCCTTCAGCTGGATCCTCGA | GTAGAGAGGGGAGCGGGTAT | Start: 641 |
| Tm | 60.033 | 59.887 | End: 757 |
| Size | 20 | 20 | 117 |
| Primer 3 details |
|---|
| Sequence | CATACCCGCTCCCCTCTCTA | CGAAGGCTCGTGAAGTCCTT | Start: 737 |
| Tm | 59.887 | 60.038 | End: 990 |
| Size | 20 | 20 | 254 |
| Primer 4 details |
|---|
| Sequence | ATACCCGCTCCCCTCTCTAC | CGAAGGCTCGTGAAGTCCTT | Start: 738 |
| Tm | 59.887 | 60.038 | End: 990 |
| Size | 20 | 20 | 253 |
| Primer 5 details |
|---|
| Sequence | GCTAACCGAAAGTTGCGACC | TCGAGGATCCAGCTGAAGGA | Start: 438 |
| Tm | 59.835 | 60.033 | End: 660 |
| Size | 20 | 20 | 223 |
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