ssr_primer
Primer Details for TRINITY_DN12266_c4_g1_i12 |
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| (A)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCGGAATGGAGGTGGGTTAA | GCTGACGGGATTCGCAATTC | Start: 3837 |
| Tm | 60.035 | 59.972 | End: 4083 |
| Size | 20 | 20 | 247 |
| Primer 2 details |
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| Sequence | GGGAAAAAGATGGGGCAGGA | GTTGCGTTGGCTGCTTGTAA | Start: 3359 |
| Tm | 59.959 | 59.97 | End: 3512 |
| Size | 20 | 20 | 154 |
| Primer 3 details |
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| Sequence | GGGAAAAAGATGGGGCAGGA | TTGCTTCGGATGATGGGCTT | Start: 3359 |
| Tm | 59.959 | 60.034 | End: 3574 |
| Size | 20 | 20 | 216 |
| Primer 4 details |
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| Sequence | GCAGCAATCTTCTCATGCCG | TGAGCCATGCTGCATAGAGG | Start: 2228 |
| Tm | 59.971 | 59.892 | End: 2356 |
| Size | 20 | 20 | 129 |
| Primer 5 details |
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| Sequence | TTACAAGCAGCCAACGCAAC | TGCTTCTTGTGCTGCTGAGA | Start: 3493 |
| Tm | 59.97 | 59.893 | End: 3639 |
| Size | 20 | 20 | 147 |
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