Primer Details for TRINITY_DN12264_c7_g1_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCATTTGGCCCCTTTTTCTCA | TGTGTACGTTGTACACTTTTAATGT | Start: 1 |
| Tm | 59.023 | 57.463 | End: 208 |
| Size | 21 | 25 | 208 |
| Primer 2 details |
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| Sequence | CCATTTGGCCCCTTTTTCTCAG | TGTGTACGTTGTACACTTTTAATGT | Start: 1 |
| Tm | 60.028 | 57.463 | End: 208 |
| Size | 22 | 25 | 208 |
| Primer 3 details |
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| Sequence | CCATTTGGCCCCTTTTTCTCA | AGTTTATGTGTACGTTGTACACTTT | Start: 1 |
| Tm | 59.023 | 57.225 | End: 214 |
| Size | 21 | 25 | 214 |
| Primer 4 details |
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| Sequence | CCATTTGGCCCCTTTTTCTCA | AAGTTTATGTGTACGTTGTACACTT | Start: 1 |
| Tm | 59.023 | 57.225 | End: 215 |
| Size | 21 | 25 | 215 |
| Primer 5 details |
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| Sequence | CCATTTGGCCCCTTTTTCTCA | AAAGTTTATGTGTACGTTGTACACT | Start: 1 |
| Tm | 59.023 | 57.225 | End: 216 |
| Size | 21 | 25 | 216 |
