ssr_primer
Primer Details for TRINITY_DN12262_c1_g2_i15 |
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| (GAG)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCGAACGTGAGACCAAGAC | TGCTGGCGTTGCTTTTCTTC | Start: 100 |
| Tm | 59.968 | 59.969 | End: 218 |
| Size | 20 | 20 | 119 |
| Primer 2 details |
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| Sequence | GCGAAGACCACAGAGACCAA | TGCTGGCGTTGCTTTTCTTC | Start: 47 |
| Tm | 59.966 | 59.969 | End: 218 |
| Size | 20 | 20 | 172 |
| Primer 3 details |
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| Sequence | TGGTCCGACAGTGTTCATGG | TGCTGGCGTTGCTTTTCTTC | Start: 28 |
| Tm | 59.965 | 59.969 | End: 218 |
| Size | 20 | 20 | 191 |
| Primer 4 details |
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| Sequence | GGGGGTTAAGCTCAGACCAC | GTTGTTGCGGTTCCTGGTTC | Start: 3916 |
| Tm | 60.035 | 59.97 | End: 4168 |
| Size | 20 | 20 | 253 |
| Primer 5 details |
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| Sequence | GGGGGTTAAGCTCAGACCAC | CAAGTTGTTGCGGTTCCTGG | Start: 3916 |
| Tm | 60.035 | 59.969 | End: 4171 |
| Size | 20 | 20 | 256 |
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