Primer Details for TRINITY_DN12255_c1_g1_i1 |
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| (AT)7: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCTCTTTTATTTGACTTCAACATGGT | CGCAACGTTTAGAAATGCATGG | Start: 241 |
| Tm | 59.494 | 59.406 | End: 358 |
| Size | 27 | 22 | 118 |
| Primer 2 details |
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| Sequence | CCTCTTTTATTTGACTTCAACATGGT | CGCAACGTTTAGAAATGCATGG | Start: 242 |
| Tm | 58.339 | 59.406 | End: 358 |
| Size | 26 | 22 | 117 |
| Primer 3 details |
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| Sequence | TCCTCTTTTATTTGACTTCAACATGG | CGCAACGTTTAGAAATGCATGG | Start: 241 |
| Tm | 58.115 | 59.406 | End: 358 |
| Size | 26 | 22 | 118 |
| Primer 4 details |
|---|
| Sequence | CCTCTTTTATTTGACTTCAACATGGTT | CGCAACGTTTAGAAATGCATGG | Start: 242 |
| Tm | 58.856 | 59.406 | End: 358 |
| Size | 27 | 22 | 117 |
| Primer 5 details |
|---|
| Sequence | TCCTCTTTTATTTGACTTCAACATGGT | ACGCAACGTTTAGAAATGCATG | Start: 241 |
| Tm | 59.494 | 58.766 | End: 359 |
| Size | 27 | 22 | 119 |
