ssr_primer
Primer Details for TRINITY_DN12250_c0_g1_i1 |
|---|
| (T)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTGCTCTTGCCTTCCCTTA | GGGGAATCCAGCAGCACTAA | Start: 408 |
| Tm | 60.035 | 59.744 | End: 542 |
| Size | 20 | 20 | 135 |
| Primer 2 details |
|---|
| Sequence | ACTTCCATTGCTGCTGCTCT | GGGGAATCCAGCAGCACTAA | Start: 396 |
| Tm | 59.962 | 59.744 | End: 542 |
| Size | 20 | 20 | 147 |
| Primer 3 details |
|---|
| Sequence | CCACGGCGCTATTAGTGACT | ATGGCGTCGAGGACAACAAA | Start: 1006 |
| Tm | 59.898 | 60.25 | End: 1180 |
| Size | 20 | 20 | 175 |
| Primer 4 details |
|---|
| Sequence | CCACGGCGCTATTAGTGACT | AATGGCGTCGAGGACAACAA | Start: 1006 |
| Tm | 59.898 | 60.25 | End: 1181 |
| Size | 20 | 20 | 176 |
| Primer 5 details |
|---|
| Sequence | CCACGGCGCTATTAGTGACT | AACAATGGCGTCGAGGACAA | Start: 1006 |
| Tm | 59.898 | 60.25 | End: 1184 |
| Size | 20 | 20 | 179 |
(TTC)7: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTGCTCTTGCCTTCCCTTA | GGGGAATCCAGCAGCACTAA | Start: 408 |
| Tm | 60.035 | 59.744 | End: 542 |
| Size | 20 | 20 | 135 |
| Primer 2 details |
|---|
| Sequence | ACTTCCATTGCTGCTGCTCT | GGGGAATCCAGCAGCACTAA | Start: 396 |
| Tm | 59.962 | 59.744 | End: 542 |
| Size | 20 | 20 | 147 |
| Primer 3 details |
|---|
| Sequence | CCACGGCGCTATTAGTGACT | ATGGCGTCGAGGACAACAAA | Start: 1006 |
| Tm | 59.898 | 60.25 | End: 1180 |
| Size | 20 | 20 | 175 |
| Primer 4 details |
|---|
| Sequence | CCACGGCGCTATTAGTGACT | AATGGCGTCGAGGACAACAA | Start: 1006 |
| Tm | 59.898 | 60.25 | End: 1181 |
| Size | 20 | 20 | 176 |
| Primer 5 details |
|---|
| Sequence | CCACGGCGCTATTAGTGACT | AACAATGGCGTCGAGGACAA | Start: 1006 |
| Tm | 59.898 | 60.25 | End: 1184 |
| Size | 20 | 20 | 179 |
(A)15: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTGCTCTTGCCTTCCCTTA | GGGGAATCCAGCAGCACTAA | Start: 408 |
| Tm | 60.035 | 59.744 | End: 542 |
| Size | 20 | 20 | 135 |
| Primer 2 details |
|---|
| Sequence | ACTTCCATTGCTGCTGCTCT | GGGGAATCCAGCAGCACTAA | Start: 396 |
| Tm | 59.962 | 59.744 | End: 542 |
| Size | 20 | 20 | 147 |
| Primer 3 details |
|---|
| Sequence | CCACGGCGCTATTAGTGACT | ATGGCGTCGAGGACAACAAA | Start: 1006 |
| Tm | 59.898 | 60.25 | End: 1180 |
| Size | 20 | 20 | 175 |
| Primer 4 details |
|---|
| Sequence | CCACGGCGCTATTAGTGACT | AATGGCGTCGAGGACAACAA | Start: 1006 |
| Tm | 59.898 | 60.25 | End: 1181 |
| Size | 20 | 20 | 176 |
| Primer 5 details |
|---|
| Sequence | CCACGGCGCTATTAGTGACT | AACAATGGCGTCGAGGACAA | Start: 1006 |
| Tm | 59.898 | 60.25 | End: 1184 |
| Size | 20 | 20 | 179 |
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