ssr_primer
Primer Details for TRINITY_DN12249_c0_g1_i3 |
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| (T)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAACCCCCTCTCTGGCATTC | CAACTCCCACTTCCCCATCC | Start: 2960 |
| Tm | 60.107 | 60.034 | End: 3177 |
| Size | 20 | 20 | 218 |
| Primer 2 details |
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| Sequence | TGCTAATGCGTCCATTGGGT | TTCTGGTGAGGGCTGTCAAC | Start: 1880 |
| Tm | 60.035 | 59.891 | End: 1992 |
| Size | 20 | 20 | 113 |
| Primer 3 details |
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| Sequence | GTTGACAGCCCTCACCAGAA | TCGAAGCTGGGAGTCCCTTA | Start: 1973 |
| Tm | 59.891 | 59.959 | End: 2205 |
| Size | 20 | 20 | 233 |
| Primer 4 details |
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| Sequence | GAACCCCCTCTCTGGCATTC | TGCTTCAACTCCCACTTCCC | Start: 2960 |
| Tm | 60.107 | 59.89 | End: 3182 |
| Size | 20 | 20 | 223 |
| Primer 5 details |
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| Sequence | GTTGACAGCCCTCACCAGAA | GAAGCTGGGAGTCCCTTACG | Start: 1973 |
| Tm | 59.891 | 59.824 | End: 2203 |
| Size | 20 | 20 | 231 |
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