ssr_primer
Primer Details for TRINITY_DN12248_c1_g4_i4 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGAAAGAACGCGGATGTCA | GGAGGGAGATATGCAGCCAC | Start: 5784 |
| Tm | 59.966 | 59.964 | End: 6040 |
| Size | 20 | 20 | 257 |
| Primer 2 details |
|---|
| Sequence | AAGAACGCGGATGTCAACCT | GGAGGGAGATATGCAGCCAC | Start: 5788 |
| Tm | 59.965 | 59.964 | End: 6040 |
| Size | 20 | 20 | 253 |
| Primer 3 details |
|---|
| Sequence | AACGCGGATGTCAACCTCAT | GGAGGGAGATATGCAGCCAC | Start: 5791 |
| Tm | 60.036 | 59.964 | End: 6040 |
| Size | 20 | 20 | 250 |
| Primer 4 details |
|---|
| Sequence | AACCAAAGCCAGCTCTCCTC | AGCGAATCCTGGGAGTGTTG | Start: 1339 |
| Tm | 59.963 | 60.036 | End: 1596 |
| Size | 20 | 20 | 258 |
| Primer 5 details |
|---|
| Sequence | GTGGCTGCATATCTCCCTCC | GGGGTGGTAGCACAATCCAA | Start: 6021 |
| Tm | 59.964 | 59.961 | End: 6140 |
| Size | 20 | 20 | 120 |
(A)13gatttgaagttcactggaaaacaaaacttagtcaactatcttctacattgaatgaattgtacgattaatttcgagtagcgttcatttctgact(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGAAAGAACGCGGATGTCA | GGAGGGAGATATGCAGCCAC | Start: 5784 |
| Tm | 59.966 | 59.964 | End: 6040 |
| Size | 20 | 20 | 257 |
| Primer 2 details |
|---|
| Sequence | AAGAACGCGGATGTCAACCT | GGAGGGAGATATGCAGCCAC | Start: 5788 |
| Tm | 59.965 | 59.964 | End: 6040 |
| Size | 20 | 20 | 253 |
| Primer 3 details |
|---|
| Sequence | AACGCGGATGTCAACCTCAT | GGAGGGAGATATGCAGCCAC | Start: 5791 |
| Tm | 60.036 | 59.964 | End: 6040 |
| Size | 20 | 20 | 250 |
| Primer 4 details |
|---|
| Sequence | AACCAAAGCCAGCTCTCCTC | AGCGAATCCTGGGAGTGTTG | Start: 1339 |
| Tm | 59.963 | 60.036 | End: 1596 |
| Size | 20 | 20 | 258 |
| Primer 5 details |
|---|
| Sequence | GTGGCTGCATATCTCCCTCC | GGGGTGGTAGCACAATCCAA | Start: 6021 |
| Tm | 59.964 | 59.961 | End: 6140 |
| Size | 20 | 20 | 120 |
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