ssr_primer
Primer Details for TRINITY_DN12236_c1_g1_i3 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTCCTCCTTCTCCTCCCAA | GGAGGAGAATCGGCTATGGC | Start: 3760 |
| Tm | 59.957 | 60.037 | End: 3911 |
| Size | 20 | 20 | 152 |
| Primer 2 details |
|---|
| Sequence | AACCCGTCACCAAAGCTTCA | GGAGGAGAATCGGCTATGGC | Start: 3689 |
| Tm | 60.107 | 60.037 | End: 3911 |
| Size | 20 | 20 | 223 |
| Primer 3 details |
|---|
| Sequence | TGAACCCGTCACCAAAGCTT | GGAGGAGAATCGGCTATGGC | Start: 3687 |
| Tm | 60.107 | 60.037 | End: 3911 |
| Size | 20 | 20 | 225 |
| Primer 4 details |
|---|
| Sequence | ACATTAGGGAGGTTGCACCG | CGTCTCCTTCCAAGATGCGT | Start: 2998 |
| Tm | 60.035 | 60.109 | End: 3215 |
| Size | 20 | 20 | 218 |
| Primer 5 details |
|---|
| Sequence | GCACCGAGTGGATGGAAAGA | CGTCTCCTTCCAAGATGCGT | Start: 3012 |
| Tm | 60.036 | 60.109 | End: 3215 |
| Size | 20 | 20 | 204 |
(AGT)5tgtcg(T)18: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTCCTCCTTCTCCTCCCAA | GGAGGAGAATCGGCTATGGC | Start: 3760 |
| Tm | 59.957 | 60.037 | End: 3911 |
| Size | 20 | 20 | 152 |
| Primer 2 details |
|---|
| Sequence | AACCCGTCACCAAAGCTTCA | GGAGGAGAATCGGCTATGGC | Start: 3689 |
| Tm | 60.107 | 60.037 | End: 3911 |
| Size | 20 | 20 | 223 |
| Primer 3 details |
|---|
| Sequence | TGAACCCGTCACCAAAGCTT | GGAGGAGAATCGGCTATGGC | Start: 3687 |
| Tm | 60.107 | 60.037 | End: 3911 |
| Size | 20 | 20 | 225 |
| Primer 4 details |
|---|
| Sequence | ACATTAGGGAGGTTGCACCG | CGTCTCCTTCCAAGATGCGT | Start: 2998 |
| Tm | 60.035 | 60.109 | End: 3215 |
| Size | 20 | 20 | 218 |
| Primer 5 details |
|---|
| Sequence | GCACCGAGTGGATGGAAAGA | CGTCTCCTTCCAAGATGCGT | Start: 3012 |
| Tm | 60.036 | 60.109 | End: 3215 |
| Size | 20 | 20 | 204 |
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