Primer Details for TRINITY_DN12234_c3_g2_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCTTGTACACTTCTTGGTCTGT | TGAAAGGAGACATCACATTCACTCT | Start: 36 |
| Tm | 60.118 | 59.988 | End: 258 |
| Size | 23 | 25 | 223 |
| Primer 2 details |
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| Sequence | TGCTTGTACACTTCTTGGTCTGT | TGAAAGGAGACATCACATTCACT | Start: 36 |
| Tm | 60.118 | 57.767 | End: 258 |
| Size | 23 | 23 | 223 |
| Primer 3 details |
|---|
| Sequence | TGCTTGTACACTTCTTGGTCTGT | TGAAAGGAGACATCACATTCACTC | Start: 36 |
| Tm | 60.118 | 58.759 | End: 258 |
| Size | 23 | 24 | 223 |
| Primer 4 details |
|---|
| Sequence | TGCTTGTACACTTCTTGGTCTG | TGAAAGGAGACATCACATTCACTCT | Start: 36 |
| Tm | 58.533 | 59.988 | End: 258 |
| Size | 22 | 25 | 223 |
| Primer 5 details |
|---|
| Sequence | GCTTGTACACTTCTTGGTCTGT | TGAAAGGAGACATCACATTCACTCT | Start: 37 |
| Tm | 58.532 | 59.988 | End: 258 |
| Size | 22 | 25 | 222 |
