ssr_primer
Primer Details for TRINITY_DN12231_c3_g1_i10 |
|---|
| (A)27: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTGGTTCCTGGGTTTGCAA | CCACACACAACGCCTTCTTG | Start: 1474 |
| Tm | 60.033 | 59.971 | End: 1646 |
| Size | 20 | 20 | 173 |
| Primer 2 details |
|---|
| Sequence | AAGTGGTTCCTGGGTTTGCA | CCACACACAACGCCTTCTTG | Start: 1473 |
| Tm | 60.033 | 59.971 | End: 1646 |
| Size | 20 | 20 | 174 |
| Primer 3 details |
|---|
| Sequence | AAGCTGCAAATGCCTTGGTG | ACTGAAGTCGAGGGTTGCTG | Start: 2801 |
| Tm | 59.966 | 59.966 | End: 2960 |
| Size | 20 | 20 | 160 |
| Primer 4 details |
|---|
| Sequence | CAAGCTGCAAATGCCTTGGT | ACTGAAGTCGAGGGTTGCTG | Start: 2800 |
| Tm | 59.966 | 59.966 | End: 2960 |
| Size | 20 | 20 | 161 |
| Primer 5 details |
|---|
| Sequence | AAGATGAGCTTGCCTGGGTC | TCGTTGCAGAGCCATCCAAT | Start: 773 |
| Tm | 60.035 | 60.035 | End: 995 |
| Size | 20 | 20 | 223 |
(AAT)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTGGTTCCTGGGTTTGCAA | CCACACACAACGCCTTCTTG | Start: 1474 |
| Tm | 60.033 | 59.971 | End: 1646 |
| Size | 20 | 20 | 173 |
| Primer 2 details |
|---|
| Sequence | AAGTGGTTCCTGGGTTTGCA | CCACACACAACGCCTTCTTG | Start: 1473 |
| Tm | 60.033 | 59.971 | End: 1646 |
| Size | 20 | 20 | 174 |
| Primer 3 details |
|---|
| Sequence | AAGCTGCAAATGCCTTGGTG | ACTGAAGTCGAGGGTTGCTG | Start: 2801 |
| Tm | 59.966 | 59.966 | End: 2960 |
| Size | 20 | 20 | 160 |
| Primer 4 details |
|---|
| Sequence | CAAGCTGCAAATGCCTTGGT | ACTGAAGTCGAGGGTTGCTG | Start: 2800 |
| Tm | 59.966 | 59.966 | End: 2960 |
| Size | 20 | 20 | 161 |
| Primer 5 details |
|---|
| Sequence | AAGATGAGCTTGCCTGGGTC | TCGTTGCAGAGCCATCCAAT | Start: 773 |
| Tm | 60.035 | 60.035 | End: 995 |
| Size | 20 | 20 | 223 |
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