ssr_primer
Primer Details for TRINITY_DN12229_c1_g6_i1 |
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| (T)17: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCTGTCGAGCGATCCGTTC | AATTACTCGACGCGGCTAGG | Start: 1126 |
| Tm | 59.97 | 59.97 | End: 1378 |
| Size | 20 | 20 | 253 |
| Primer 2 details |
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| Sequence | GAATCTGTCGAGCGATCCGT | AATTACTCGACGCGGCTAGG | Start: 1124 |
| Tm | 59.97 | 59.97 | End: 1378 |
| Size | 20 | 20 | 255 |
| Primer 3 details |
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| Sequence | CCTAGCCGCGTCGAGTAATT | CGGCTGAGATTTGGGGAGTT | Start: 1359 |
| Tm | 59.97 | 60.035 | End: 1488 |
| Size | 20 | 20 | 130 |
| Primer 4 details |
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| Sequence | CCTCCAACATCCTCACCACC | TGGGAAGGAGAAAGAACGCC | Start: 553 |
| Tm | 60.035 | 59.964 | End: 789 |
| Size | 20 | 20 | 237 |
| Primer 5 details |
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| Sequence | CCCTCCAACATCCTCACCAC | TGGGAAGGAGAAAGAACGCC | Start: 552 |
| Tm | 60.035 | 59.964 | End: 789 |
| Size | 20 | 20 | 238 |
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