ssr_primer
Primer Details for TRINITY_DN12229_c0_g1_i7 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGGCGGATGCTATGGTTTT | GCAGACCCCTTAGTGTCACC | Start: 1172 |
| Tm | 60.034 | 60.036 | End: 1349 |
| Size | 20 | 20 | 178 |
| Primer 2 details |
|---|
| Sequence | ACTGATGGGCGGATGCTATG | GCAGACCCCTTAGTGTCACC | Start: 1167 |
| Tm | 59.964 | 60.036 | End: 1349 |
| Size | 20 | 20 | 183 |
| Primer 3 details |
|---|
| Sequence | TTCTCTCTCGCGCCCAAATT | CGCGAACATTAGACCTCGGA | Start: 187 |
| Tm | 60.036 | 59.9 | End: 321 |
| Size | 20 | 20 | 135 |
| Primer 4 details |
|---|
| Sequence | TTCTCTCTCGCGCCCAAATT | TCGCGAACATTAGACCTCGG | Start: 187 |
| Tm | 60.036 | 59.9 | End: 322 |
| Size | 20 | 20 | 136 |
| Primer 5 details |
|---|
| Sequence | TGGGCGGATGCTATGGTTTT | ACCCCTTAGTGTCACCCTGT | Start: 1172 |
| Tm | 60.034 | 60.105 | End: 1345 |
| Size | 20 | 20 | 174 |
(CT)25: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGGCGGATGCTATGGTTTT | GCAGACCCCTTAGTGTCACC | Start: 1172 |
| Tm | 60.034 | 60.036 | End: 1349 |
| Size | 20 | 20 | 178 |
| Primer 2 details |
|---|
| Sequence | ACTGATGGGCGGATGCTATG | GCAGACCCCTTAGTGTCACC | Start: 1167 |
| Tm | 59.964 | 60.036 | End: 1349 |
| Size | 20 | 20 | 183 |
| Primer 3 details |
|---|
| Sequence | TTCTCTCTCGCGCCCAAATT | CGCGAACATTAGACCTCGGA | Start: 187 |
| Tm | 60.036 | 59.9 | End: 321 |
| Size | 20 | 20 | 135 |
| Primer 4 details |
|---|
| Sequence | TTCTCTCTCGCGCCCAAATT | TCGCGAACATTAGACCTCGG | Start: 187 |
| Tm | 60.036 | 59.9 | End: 322 |
| Size | 20 | 20 | 136 |
| Primer 5 details |
|---|
| Sequence | TGGGCGGATGCTATGGTTTT | ACCCCTTAGTGTCACCCTGT | Start: 1172 |
| Tm | 60.034 | 60.105 | End: 1345 |
| Size | 20 | 20 | 174 |
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