ssr_primer
Primer Details for TRINITY_DN12225_c1_g3_i1 |
|---|
| (AT)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGTACCCACCACACACCTC | TACATCGGTGGAGCAGAGGA | Start: 1342 |
| Tm | 59.965 | 60.034 | End: 1457 |
| Size | 20 | 20 | 116 |
| Primer 2 details |
|---|
| Sequence | CTGTACCCACCACACACCTC | CGGGGAATACATCGGTGGAG | Start: 1342 |
| Tm | 59.965 | 59.966 | End: 1464 |
| Size | 20 | 20 | 123 |
| Primer 3 details |
|---|
| Sequence | TCCTCTGCTCCACCGATGTA | ACCGTCCGTTCAATACTCCG | Start: 1438 |
| Tm | 60.034 | 59.828 | End: 1598 |
| Size | 20 | 20 | 161 |
| Primer 4 details |
|---|
| Sequence | CTCCACCGATGTATTCCCCG | ACCGTCCGTTCAATACTCCG | Start: 1445 |
| Tm | 59.966 | 59.828 | End: 1598 |
| Size | 20 | 20 | 154 |
| Primer 5 details |
|---|
| Sequence | CGGAGTATTGAACGGACGGT | TCTCCCGTCCGAGAGTCAAT | Start: 1579 |
| Tm | 59.828 | 60.034 | End: 1791 |
| Size | 20 | 20 | 213 |
(TTG)8: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGTACCCACCACACACCTC | TACATCGGTGGAGCAGAGGA | Start: 1342 |
| Tm | 59.965 | 60.034 | End: 1457 |
| Size | 20 | 20 | 116 |
| Primer 2 details |
|---|
| Sequence | CTGTACCCACCACACACCTC | CGGGGAATACATCGGTGGAG | Start: 1342 |
| Tm | 59.965 | 59.966 | End: 1464 |
| Size | 20 | 20 | 123 |
| Primer 3 details |
|---|
| Sequence | TCCTCTGCTCCACCGATGTA | ACCGTCCGTTCAATACTCCG | Start: 1438 |
| Tm | 60.034 | 59.828 | End: 1598 |
| Size | 20 | 20 | 161 |
| Primer 4 details |
|---|
| Sequence | CTCCACCGATGTATTCCCCG | ACCGTCCGTTCAATACTCCG | Start: 1445 |
| Tm | 59.966 | 59.828 | End: 1598 |
| Size | 20 | 20 | 154 |
| Primer 5 details |
|---|
| Sequence | CGGAGTATTGAACGGACGGT | TCTCCCGTCCGAGAGTCAAT | Start: 1579 |
| Tm | 59.828 | 60.034 | End: 1791 |
| Size | 20 | 20 | 213 |
(A)50(AAG)7*: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGTACCCACCACACACCTC | TACATCGGTGGAGCAGAGGA | Start: 1342 |
| Tm | 59.965 | 60.034 | End: 1457 |
| Size | 20 | 20 | 116 |
| Primer 2 details |
|---|
| Sequence | CTGTACCCACCACACACCTC | CGGGGAATACATCGGTGGAG | Start: 1342 |
| Tm | 59.965 | 59.966 | End: 1464 |
| Size | 20 | 20 | 123 |
| Primer 3 details |
|---|
| Sequence | TCCTCTGCTCCACCGATGTA | ACCGTCCGTTCAATACTCCG | Start: 1438 |
| Tm | 60.034 | 59.828 | End: 1598 |
| Size | 20 | 20 | 161 |
| Primer 4 details |
|---|
| Sequence | CTCCACCGATGTATTCCCCG | ACCGTCCGTTCAATACTCCG | Start: 1445 |
| Tm | 59.966 | 59.828 | End: 1598 |
| Size | 20 | 20 | 154 |
| Primer 5 details |
|---|
| Sequence | CGGAGTATTGAACGGACGGT | TCTCCCGTCCGAGAGTCAAT | Start: 1579 |
| Tm | 59.828 | 60.034 | End: 1791 |
| Size | 20 | 20 | 213 |
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