ssr_primer
Primer Details for TRINITY_DN12218_c2_g1_i1 |
|---|
| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGCAGCACGACAAAAACCC | CCGTTGTTGATGGCGTTGTT | Start: 1370 |
| Tm | 59.968 | 59.97 | End: 1541 |
| Size | 20 | 20 | 172 |
| Primer 2 details |
|---|
| Sequence | AGTTAGTGCTCCCTCGAGGT | ACCAACATTCCCACGAGCTT | Start: 739 |
| Tm | 59.96 | 59.89 | End: 954 |
| Size | 20 | 20 | 216 |
| Primer 3 details |
|---|
| Sequence | ATGCAGCACGACAAAAACCC | TGAGGCACGTAAATCTCCCG | Start: 1370 |
| Tm | 59.968 | 59.825 | End: 1558 |
| Size | 20 | 20 | 189 |
| Primer 4 details |
|---|
| Sequence | ATGCAGCACGACAAAAACCC | GAGGCACGTAAATCTCCCGT | Start: 1370 |
| Tm | 59.968 | 59.825 | End: 1557 |
| Size | 20 | 20 | 188 |
| Primer 5 details |
|---|
| Sequence | AAGCTCGTGGGAATGTTGGT | GCCCACATCTTCCTCCTTCC | Start: 935 |
| Tm | 59.89 | 60.107 | End: 1111 |
| Size | 20 | 20 | 177 |
(AAG)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGCAGCACGACAAAAACCC | CCGTTGTTGATGGCGTTGTT | Start: 1370 |
| Tm | 59.968 | 59.97 | End: 1541 |
| Size | 20 | 20 | 172 |
| Primer 2 details |
|---|
| Sequence | AGTTAGTGCTCCCTCGAGGT | ACCAACATTCCCACGAGCTT | Start: 739 |
| Tm | 59.96 | 59.89 | End: 954 |
| Size | 20 | 20 | 216 |
| Primer 3 details |
|---|
| Sequence | ATGCAGCACGACAAAAACCC | TGAGGCACGTAAATCTCCCG | Start: 1370 |
| Tm | 59.968 | 59.825 | End: 1558 |
| Size | 20 | 20 | 189 |
| Primer 4 details |
|---|
| Sequence | ATGCAGCACGACAAAAACCC | GAGGCACGTAAATCTCCCGT | Start: 1370 |
| Tm | 59.968 | 59.825 | End: 1557 |
| Size | 20 | 20 | 188 |
| Primer 5 details |
|---|
| Sequence | AAGCTCGTGGGAATGTTGGT | GCCCACATCTTCCTCCTTCC | Start: 935 |
| Tm | 59.89 | 60.107 | End: 1111 |
| Size | 20 | 20 | 177 |
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