ssr_primer
Primer Details for TRINITY_DN12208_c1_g1_i7 |
|---|
| (A)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTAAGGTTGGCTAGCCTGGC | CTCGACCATAGACGCACTCC | Start: 576 |
| Tm | 60.034 | 59.97 | End: 689 |
| Size | 20 | 20 | 114 |
| Primer 2 details |
|---|
| Sequence | TCTGCACGCTAGAAACTGCA | CCGGTTCTTTGCCAACCAAG | Start: 1012 |
| Tm | 59.967 | 59.968 | End: 1207 |
| Size | 20 | 20 | 196 |
| Primer 3 details |
|---|
| Sequence | CTTGGTTGGCAAAGAACCGG | ATGACTGTTCTTCGTGCGGT | Start: 1188 |
| Tm | 59.968 | 59.966 | End: 1437 |
| Size | 20 | 20 | 250 |
| Primer 4 details |
|---|
| Sequence | ACCGCACGAAGAACAGTCAT | AATCCCTAAACCCTGCCTGC | Start: 1418 |
| Tm | 59.966 | 60.033 | End: 1552 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | ACCAACCGACCAGACATGAC | CATGGGAATGGCTCCTGTGT | Start: 3185 |
| Tm | 59.965 | 60.034 | End: 3367 |
| Size | 20 | 20 | 183 |
(GA)9aagagaaagagaaca(AG)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTAAGGTTGGCTAGCCTGGC | CTCGACCATAGACGCACTCC | Start: 576 |
| Tm | 60.034 | 59.97 | End: 689 |
| Size | 20 | 20 | 114 |
| Primer 2 details |
|---|
| Sequence | TCTGCACGCTAGAAACTGCA | CCGGTTCTTTGCCAACCAAG | Start: 1012 |
| Tm | 59.967 | 59.968 | End: 1207 |
| Size | 20 | 20 | 196 |
| Primer 3 details |
|---|
| Sequence | CTTGGTTGGCAAAGAACCGG | ATGACTGTTCTTCGTGCGGT | Start: 1188 |
| Tm | 59.968 | 59.966 | End: 1437 |
| Size | 20 | 20 | 250 |
| Primer 4 details |
|---|
| Sequence | ACCGCACGAAGAACAGTCAT | AATCCCTAAACCCTGCCTGC | Start: 1418 |
| Tm | 59.966 | 60.033 | End: 1552 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | ACCAACCGACCAGACATGAC | CATGGGAATGGCTCCTGTGT | Start: 3185 |
| Tm | 59.965 | 60.034 | End: 3367 |
| Size | 20 | 20 | 183 |
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