ssr_primer
Primer Details for TRINITY_DN12208_c0_g1_i1 |
|---|
| (A)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGCATTGGCTGGAAGAACA | GGCCAAGCTATTGCGACATG | Start: 1436 |
| Tm | 59.887 | 59.97 | End: 1618 |
| Size | 20 | 20 | 183 |
| Primer 2 details |
|---|
| Sequence | TGTCTGGATTCTGGAGGGGT | GGCCAAGCTATTGCGACATG | Start: 1409 |
| Tm | 59.881 | 59.97 | End: 1618 |
| Size | 20 | 20 | 210 |
| Primer 3 details |
|---|
| Sequence | GGATTCTGGAGGGGTGATGC | GGCCAAGCTATTGCGACATG | Start: 1414 |
| Tm | 60.179 | 59.97 | End: 1618 |
| Size | 20 | 20 | 205 |
| Primer 4 details |
|---|
| Sequence | GAGAGAGAGCACACACCACC | TTGGCATGGTAGAGATGGCC | Start: 779 |
| Tm | 60.038 | 59.817 | End: 1012 |
| Size | 20 | 20 | 234 |
| Primer 5 details |
|---|
| Sequence | TTCAGATCCGAACACAGCGT | GGCCAAGCTATTGCGACATG | Start: 1488 |
| Tm | 59.683 | 59.97 | End: 1618 |
| Size | 20 | 20 | 131 |
(AT)6ttacaaataaatatatatctgcaataagagagaaagagaa(AG)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGCATTGGCTGGAAGAACA | GGCCAAGCTATTGCGACATG | Start: 1436 |
| Tm | 59.887 | 59.97 | End: 1618 |
| Size | 20 | 20 | 183 |
| Primer 2 details |
|---|
| Sequence | TGTCTGGATTCTGGAGGGGT | GGCCAAGCTATTGCGACATG | Start: 1409 |
| Tm | 59.881 | 59.97 | End: 1618 |
| Size | 20 | 20 | 210 |
| Primer 3 details |
|---|
| Sequence | GGATTCTGGAGGGGTGATGC | GGCCAAGCTATTGCGACATG | Start: 1414 |
| Tm | 60.179 | 59.97 | End: 1618 |
| Size | 20 | 20 | 205 |
| Primer 4 details |
|---|
| Sequence | GAGAGAGAGCACACACCACC | TTGGCATGGTAGAGATGGCC | Start: 779 |
| Tm | 60.038 | 59.817 | End: 1012 |
| Size | 20 | 20 | 234 |
| Primer 5 details |
|---|
| Sequence | TTCAGATCCGAACACAGCGT | GGCCAAGCTATTGCGACATG | Start: 1488 |
| Tm | 59.683 | 59.97 | End: 1618 |
| Size | 20 | 20 | 131 |
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