ssr_primer
Primer Details for TRINITY_DN12206_c2_g4_i3 |
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| (AAT)9tatacaagctgcaatgaaaagggaaattaagaaaggaaagggggaaatgaaattagggaaggcagggatgtaaaaagaaaattatatatatat(TA)6aaag(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCAGCACATCAACATCGCT | GGTGGAGAAACACAAACGCC | Start: 417 |
| Tm | 60.036 | 59.97 | End: 519 |
| Size | 20 | 20 | 103 |
| Primer 2 details |
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| Sequence | GGCGTTTGTGTTTCTCCACC | AACGATGAAAACTGCACGGC | Start: 500 |
| Tm | 59.97 | 60.04 | End: 686 |
| Size | 20 | 20 | 187 |
| Primer 3 details |
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| Sequence | GTCCAAGAAGGTGGTCGAGG | TGATCCACACCACATCCACG | Start: 833 |
| Tm | 60.037 | 60.036 | End: 1000 |
| Size | 20 | 20 | 168 |
| Primer 4 details |
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| Sequence | GTAAAGGGTTCGTGCTCCGA | AGCGATGTTGATGTGCTGGA | Start: 204 |
| Tm | 60.038 | 60.036 | End: 436 |
| Size | 20 | 20 | 233 |
| Primer 5 details |
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| Sequence | GTAAAGGGTTCGTGCTCCGA | GCTGAGAGAGCCACGTTTCT | Start: 204 |
| Tm | 60.038 | 60.038 | End: 470 |
| Size | 20 | 20 | 267 |
(TGT)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCAGCACATCAACATCGCT | GGTGGAGAAACACAAACGCC | Start: 417 |
| Tm | 60.036 | 59.97 | End: 519 |
| Size | 20 | 20 | 103 |
| Primer 2 details |
|---|
| Sequence | GGCGTTTGTGTTTCTCCACC | AACGATGAAAACTGCACGGC | Start: 500 |
| Tm | 59.97 | 60.04 | End: 686 |
| Size | 20 | 20 | 187 |
| Primer 3 details |
|---|
| Sequence | GTCCAAGAAGGTGGTCGAGG | TGATCCACACCACATCCACG | Start: 833 |
| Tm | 60.037 | 60.036 | End: 1000 |
| Size | 20 | 20 | 168 |
| Primer 4 details |
|---|
| Sequence | GTAAAGGGTTCGTGCTCCGA | AGCGATGTTGATGTGCTGGA | Start: 204 |
| Tm | 60.038 | 60.036 | End: 436 |
| Size | 20 | 20 | 233 |
| Primer 5 details |
|---|
| Sequence | GTAAAGGGTTCGTGCTCCGA | GCTGAGAGAGCCACGTTTCT | Start: 204 |
| Tm | 60.038 | 60.038 | End: 470 |
| Size | 20 | 20 | 267 |
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