ssr_primer
Primer Details for TRINITY_DN12188_c2_g8_i1 |
|---|
| (CTT)5cctcttcctcttcctcc(TCT)8: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAATCAGCCGCCGGTCTTTA | ACCCTTATGGCTGTTCGGTG | Start: 493 |
| Tm | 60.036 | 60.035 | End: 746 |
| Size | 20 | 20 | 254 |
| Primer 2 details |
|---|
| Sequence | CAGTGCCGGAAACTCATCCT | ACCCTTATGGCTGTTCGGTG | Start: 550 |
| Tm | 60.036 | 60.035 | End: 746 |
| Size | 20 | 20 | 197 |
| Primer 3 details |
|---|
| Sequence | AAATCAGCCGCCGGTCTTTA | CGGTGGAGGCGATGGAATAA | Start: 493 |
| Tm | 60.036 | 59.894 | End: 731 |
| Size | 20 | 20 | 239 |
| Primer 4 details |
|---|
| Sequence | CAGTGCCGGAAACTCATCCT | CGGTGGAGGCGATGGAATAA | Start: 550 |
| Tm | 60.036 | 59.894 | End: 731 |
| Size | 20 | 20 | 182 |
| Primer 5 details |
|---|
| Sequence | TCCCGGTAGCGATTTTTGCT | AATCAGCTCCCCATTAGGCG | Start: 142 |
| Tm | 60.036 | 59.891 | End: 410 |
| Size | 20 | 20 | 269 |
(A)15ggatgttttgatattttcccactcc(T)11attatttttcttgagactcaaaatgagagcgtaaattccc(A)11gaag(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAATCAGCCGCCGGTCTTTA | ACCCTTATGGCTGTTCGGTG | Start: 493 |
| Tm | 60.036 | 60.035 | End: 746 |
| Size | 20 | 20 | 254 |
| Primer 2 details |
|---|
| Sequence | CAGTGCCGGAAACTCATCCT | ACCCTTATGGCTGTTCGGTG | Start: 550 |
| Tm | 60.036 | 60.035 | End: 746 |
| Size | 20 | 20 | 197 |
| Primer 3 details |
|---|
| Sequence | AAATCAGCCGCCGGTCTTTA | CGGTGGAGGCGATGGAATAA | Start: 493 |
| Tm | 60.036 | 59.894 | End: 731 |
| Size | 20 | 20 | 239 |
| Primer 4 details |
|---|
| Sequence | CAGTGCCGGAAACTCATCCT | CGGTGGAGGCGATGGAATAA | Start: 550 |
| Tm | 60.036 | 59.894 | End: 731 |
| Size | 20 | 20 | 182 |
| Primer 5 details |
|---|
| Sequence | TCCCGGTAGCGATTTTTGCT | AATCAGCTCCCCATTAGGCG | Start: 142 |
| Tm | 60.036 | 59.891 | End: 410 |
| Size | 20 | 20 | 269 |
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