ssr_primer
Primer Details for TRINITY_DN12188_c1_g1_i3 |
|---|
| (T)18: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGAATGCCATTGAGCTTCCG | ATAATCAGGGCCAGGAGGGT | Start: 707 |
| Tm | 59.971 | 60.029 | End: 822 |
| Size | 20 | 20 | 116 |
| Primer 2 details |
|---|
| Sequence | CGAATGCCATTGAGCTTCCG | GCCAGGAGGGTTGCAAGTAT | Start: 707 |
| Tm | 59.971 | 60.034 | End: 813 |
| Size | 20 | 20 | 107 |
| Primer 3 details |
|---|
| Sequence | AGCTTGAATCCTCGAACCCG | ATAATCAGGGCCAGGAGGGT | Start: 671 |
| Tm | 60.108 | 60.029 | End: 822 |
| Size | 20 | 20 | 152 |
| Primer 4 details |
|---|
| Sequence | CGAATGCCATTGAGCTTCCG | CTTCGGTGGGGGAAGAAACA | Start: 707 |
| Tm | 59.971 | 59.89 | End: 880 |
| Size | 20 | 20 | 174 |
| Primer 5 details |
|---|
| Sequence | CGAATGCCATTGAGCTTCCG | CCTTTTCTTCGGTGGGGGAA | Start: 707 |
| Tm | 59.971 | 59.888 | End: 886 |
| Size | 20 | 20 | 180 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGAATGCCATTGAGCTTCCG | ATAATCAGGGCCAGGAGGGT | Start: 707 |
| Tm | 59.971 | 60.029 | End: 822 |
| Size | 20 | 20 | 116 |
| Primer 2 details |
|---|
| Sequence | CGAATGCCATTGAGCTTCCG | GCCAGGAGGGTTGCAAGTAT | Start: 707 |
| Tm | 59.971 | 60.034 | End: 813 |
| Size | 20 | 20 | 107 |
| Primer 3 details |
|---|
| Sequence | AGCTTGAATCCTCGAACCCG | ATAATCAGGGCCAGGAGGGT | Start: 671 |
| Tm | 60.108 | 60.029 | End: 822 |
| Size | 20 | 20 | 152 |
| Primer 4 details |
|---|
| Sequence | CGAATGCCATTGAGCTTCCG | CTTCGGTGGGGGAAGAAACA | Start: 707 |
| Tm | 59.971 | 59.89 | End: 880 |
| Size | 20 | 20 | 174 |
| Primer 5 details |
|---|
| Sequence | CGAATGCCATTGAGCTTCCG | CCTTTTCTTCGGTGGGGGAA | Start: 707 |
| Tm | 59.971 | 59.888 | End: 886 |
| Size | 20 | 20 | 180 |
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