ssr_primer
Primer Details for TRINITY_DN12185_c0_g4_i1 |
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| (A)25caag(A)15cccctcaatcattgtgatatcctcttcataatcgttgcctgaac(T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CATCTCGACTGCGTTCGGTA | GCTTCCATCAGGAGTGGCTT | Start: 799 |
| Tm | 59.902 | 60.035 | End: 1077 |
| Size | 20 | 20 | 279 |
| Primer 2 details |
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| Sequence | TCTCGACTGCGTTCGGTAAC | GCTTCCATCAGGAGTGGCTT | Start: 801 |
| Tm | 60.11 | 60.035 | End: 1077 |
| Size | 20 | 20 | 277 |
| Primer 3 details |
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| Sequence | CGGGAATCTTCCTCTTGCCA | GCTTCCATCAGGAGTGGCTT | Start: 823 |
| Tm | 59.747 | 60.035 | End: 1077 |
| Size | 20 | 20 | 255 |
| Primer 4 details |
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| Sequence | ACCGGGAATCTTCCTCTTGC | GCTTCCATCAGGAGTGGCTT | Start: 821 |
| Tm | 59.747 | 60.035 | End: 1077 |
| Size | 20 | 20 | 257 |
| Primer 5 details |
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| Sequence | CAAAGCAGTCCTCTTTGGCG | GACTCATTAGGCCCCTCAGC | Start: 1360 |
| Tm | 59.761 | 59.892 | End: 1491 |
| Size | 20 | 20 | 132 |
(CTTC)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CATCTCGACTGCGTTCGGTA | GCTTCCATCAGGAGTGGCTT | Start: 799 |
| Tm | 59.902 | 60.035 | End: 1077 |
| Size | 20 | 20 | 279 |
| Primer 2 details |
|---|
| Sequence | TCTCGACTGCGTTCGGTAAC | GCTTCCATCAGGAGTGGCTT | Start: 801 |
| Tm | 60.11 | 60.035 | End: 1077 |
| Size | 20 | 20 | 277 |
| Primer 3 details |
|---|
| Sequence | CGGGAATCTTCCTCTTGCCA | GCTTCCATCAGGAGTGGCTT | Start: 823 |
| Tm | 59.747 | 60.035 | End: 1077 |
| Size | 20 | 20 | 255 |
| Primer 4 details |
|---|
| Sequence | ACCGGGAATCTTCCTCTTGC | GCTTCCATCAGGAGTGGCTT | Start: 821 |
| Tm | 59.747 | 60.035 | End: 1077 |
| Size | 20 | 20 | 257 |
| Primer 5 details |
|---|
| Sequence | CAAAGCAGTCCTCTTTGGCG | GACTCATTAGGCCCCTCAGC | Start: 1360 |
| Tm | 59.761 | 59.892 | End: 1491 |
| Size | 20 | 20 | 132 |
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